LOCUS       sequence-114573-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse Oct4 targeting vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Ochiai H, Sugawara T, Yamamoto T
  TITLE     Simultaneous live imaging of the transcription and nuclear position 
            of specific genes.
  JOURNAL   Nucleic Acids Res. 2015 Jun 19. pii: gkv624.
  PUBMED    26092696
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jun 19. pii: gkv624."
COMMENT     Sequence Label: pTV-Oct4-TK-HMS
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    285..318
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(319..372)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ggaagccccc agcacctgcc agtaagtcat cggctcgggt acgtagacga tatcgtcgcg
       61 cgaacccagg gccaccagca gttgcgtggt ggtggttttc cccatcccgt gaggaccgtc
      121 tatataaacc cgcagtagcg tgggcatttt ctgctccagg cggacttccg tggcttcttg
      181 ctgccggcga gggcgcaacg ccgtacgtcg gttgctatgg ccgcgagaac gcgcagcctg
      241 gtcgaacgca gacgcgtgtt gatggcaggg gtacgaagcc atcaataact tcgtataatg
      301 tatgctatac gaagttatgg gaccaggatt ttcctctacg tccccgcaag ttagcaggct
      361 acctcttccc tcgtttgaat gcatgggaga gcccagagca gtgacgggaa cagagggaaa
      421 ggcctcgccc tcaggaaaag ggactgagta gagtgtggtg aagtgggggc ttccatagcc
      481 tggggtgcca aagtggggac caggaggcag tgggaaagat acagctccac ctggaaatgg
      541 ggtaccggta gcctcgtact cttctcgttg ggaatactca atacttgatc ttttgccctt
      601 ctggcgccgg ttacagaacc atactcgaac cacctaggat gggcacagag atggacatca
      661 gatcataagc tctgggatgc tgcagtcagc ggtggcggcg gtgggggtgg gggggtgggg
      721 ggggt
//
