LOCUS       sequence-114580-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Mito-7
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..89
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      117..126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(168..189)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(429..448)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcagatccgc tagcatgtcc gtcctgacgc cgctgctgct gcggggcttg acaggctcgg
       61 cccggcggct cccagtgccg cgcgccaaga tccattcgtt gggggatcca ccggtcgcca
      121 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      181 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      241 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      301 ccctcgtgac caccctgagc tggggcgtgc agtgcttcgc ccgctacccc gaccacatga
      361 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      421 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      481 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      541 acaagctgga gtacaacgcc atccacggca acgtctatat caccgccgac aagcagaaga
      601 acggcatcaa ggccaacttc ggcctcaact gcaacatcga ggacggcagc gtgcagctcg
      661 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
      721 actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
//
