LOCUS       sequence-114582-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KanR + ColE1 + PLlacO-1::RBS(st7):cfp::Asp terminator + 
            PLlacO-1::RBS(st7):psu::T1 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Hussein R, Lee TY, Lim HN
  TITLE     Quantitative characterization of gene regulation by Rho dependent 
            transcription termination.
  JOURNAL   Biochim Biophys Acta. 2015 May 14;1849(8):940-954. doi: 
            10.1016/j.bbagrm.2015.05.003.
  PUBMED    25982507
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbagrm.2015.05"; journalName: "Biochim Biophys Acta"; 
            date: "2015-05-14- 14"; volume: "1849"; issue: "8"; pages: "940-954"
COMMENT     Sequence Label: pHL 1988
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    132..148
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      777..863
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gctgtcagta aggatcctat gaagctttaa tcgtacaggg tagtacaaat aaaaaaggca
       61 cgtcagatga cgtgcctttt ttcttgtgag cagtgaattc gatccataaa tgtgagcgga
      121 taacattgac attgtgagcg gataacaaga tactcccggg taaggaggaa aaaaaatgga
      181 aagcacagcc ttacagcagg cctttgacac ctgtcagaat aacaaagcag catggctgca
      241 acgcaaaaat gagctggcag cggccgaaca ggaatatctg cggcttctgt caggagaagg
      301 cagaaacgtc agtcgcctgg acgaattacg caatattatc gaagtcagaa aatggcaggt
      361 gaatcaggcc gccggtcgtt atattcgttc gcatgaagcc gttcagcaca tcagcatccg
      421 cgaccggctg aatgatttta tgcagcagca cggcacagca ctggcggccg cactggcacc
      481 ggagctgatg ggctacagtg agctgacggc cattgcccga aactgtgcca tacagcgtgc
      541 cacagatgcc ctgcgtgaag cccttctgtc ctggcttgcg aagggtgaaa aaattaatta
      601 ttccgcacag gatagcgaca ttttaacgac catcggattc aggcctgacg tggcttcggt
      661 ggatgacagc cgtgaaaaat tcacccctgc gcagaacatg attttttcgc gtaaaagtgc
      721 gcaactggca tcacgtcagt cagtgtaagg gcccatggta cgcgtgctag aggcatcaaa
      781 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      841 acgctctcct gagtaggaca aatccgccgc cctagaccta gggcgttcgg ctgcggcgag
      901 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgca
//
