LOCUS       sequence-114586-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, N1 Cloning Vector, Excitation:505
            / 569, Emission:516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4a-CenpB-N-22
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    525..577
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      585..594
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagatccgct agccaccatg ggccccaagc ggcggcagct gacgttccgg gagaagtcgc
       61 ggatcatcca ggaggtggag gagaacccgg acctacgcaa gggcgagatc gcgcggcgct
      121 tcaacatccc gccgtccacg ctgagcacga tcctgaagaa caagcgcgcc atcctggcgt
      181 cggagcgcaa gtacggggtg gcctccacct gccgcaagac caacaagctg tctccctacg
      241 acaagctcga gggcttgctc atcgcctggt tccagcagat ccgcgccgcc ggcctgccgg
      301 tcaagggcat catcctcaag gagaaggcgc tgcgcatagc cgaggagctg ggcatggacg
      361 acttcaccgc ctccaacggc tggctggacc gcttccgccg gcgccacggc gtggtgtcct
      421 gcagcggcgt ggcccgcgcc cgcgcgcgaa acgctgcccc ccgcaccccg gcggcgcctg
      481 ccagtccggc cgcggtgccc tcggagggca gtggcgggag cactctcgag ctcaagcttc
      541 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagtg
      601 cgattaagcc agacatgagg atcaaactcc gtatggaagg caacgtaaac gggcaccact
      661 ttgtgatcga cggagatggt acaggcaagc cttatgaggg aaaacagacc atggatcttg
      721 aagtcaaaga gggcggacct ctgccttttg cctttgatat cctgaccact gcattccatt
      781 acggcaacag ggtattcgtt aaatatccag acaacataca agactatttt aagcagtcgt
      841 ttcctaaggg gtattcgtgg gaacgaagct
//
