LOCUS       sequence-114587-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, N1 Cloning Vector, Excitation:505
            / 569, Emission:516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4a-CenpB-N-22
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(119..802)
                     /codon_start=1
                     /product="fixation-resistant green-to-red photoswitchable 
                     fluorescent protein (Paez-Segala et al., 2015)"
                     /label=mEos4a
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICYA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     regulatory      799..808
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
       61 agcaagtaaa acctctacaa atgtggtatg gctgattatg atctagagtc gcggccgctt
      121 atcgtctggc attgtcaggc aatccagaat gagcaacagc atgctcatac agcttaacct
      181 tgttgtaatc tttgtcatgg cttaaaatct caatggcgtg gtccacaaag tggtagcctg
      241 gtaacttgac acccttctcc ttagctttgt aagtagttct gaagtcacat cggtaatggg
      301 catttccttc aagcaacaaa gccatatgaa tatcacccgt cagcactcca tcacgcacat
      361 acattttctc agtggagggc tcccatttca gcgtcttctt ctgcataact ggaccattgg
      421 cgggaaagtt ggtaccataa aatcgaactt tattatagaa agtgtcccct tccattgtta
      481 tgtcgtttct ggcatagcaa atgcccccgt cttcgaaagt caagcttcgt tcccacgaat
      541 accccttagg aaacgactgc ttaaaatagt cttgtatgtt gtctggatat ttaacgaata
      601 ccctgttgcc gtaatggaat gcagtggtca ggatatcaaa ggcaaaaggc agaggtccgc
      661 cctctttgac ttcaagatcc atggtctgtt ttccctcata aggcttgcct gtaccatctc
      721 cgtcgatcac aaagtggtgc ccgtttacgt tgccttccat acggagtttg atcctcatgt
      781 ctggcttaat cgcactcacc atggtggcga ccggtggatc ccgggcccgc
//
