LOCUS       sequence-11459-e        9025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Edwards SR, Wandless TJ
  TITLE     Dicistronic regulation of fluorescent proteins in the budding yeast 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2010 Apr;27(4):229-36. doi: 10.1002/yea.1744
  PUBMED    20017217
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea"; 
            journalName: "Yeast"; date: "2010-04"; volume: "27"; issue: "4"; 
            pages: "229-36"
COMMENT     Sequence Label: pGADT7-ADH700-yeCherry-pTEF1 (S.C.)-yeGFP-DHFR
FEATURES             Location/Qualifiers
     source          1..9025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..719
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     promoter        1451..1850
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             1867..2580
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1867..2580
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2590..3066
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDHVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     terminator      3453..3640
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3508..3532)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(3757..4851)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4852..5257)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(5299..5315)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5299..5315)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5312..5334)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5323..5339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5347..5353,5354..5371,5372..5377))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5392..5413
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    5468..5501
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5579..5612
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5681..5698)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5852..6440)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5932..5951)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6611..7402,7403..7471))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7234..7253
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7472..7576)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7644..7662
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7700..7722)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7822..7841
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      7858..9022
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tgcatgcctg caggatcctt ttgttgtttc cgggtgtaca atatggactt cctcttttct
       61 ggcaaccaaa cccatacatc gggattccta taataccttc gttggtctcc ctaacatgta
      121 ggtggcggag gggagatata caatagaaca gataccagac aagacataat gggctaaaca
      181 agactacacc aattacactg cctcattgat ggtggtacat aacgaactaa tactgtagcc
      241 ctagacttga tagccatcat catatcgaag tttcactacc ctttttccat ttgccatcta
      301 ttgaagtaat aataggcgca tgcaacttct tttctttttt tttcttttct ctctcccccg
      361 ttgttgtctc accatatccg caatgacaaa aaaaatgatg gaagacacta aaggaaaaaa
      421 ttaacgacaa agacagcacc aacagatgtc gttgttccag agctgatgag gggtatctcg
      481 aagcacacga aactttttcc ttccttcatt cacgcacact actctctaat gagcaacggt
      541 atacggcctt ccttccagtt acttgaattt gaaataaaaa aagtttgctg tcttgctatc
      601 aagtataaat agacctgcaa ttattaatct tttgtttcct cgtcattgtt ctcgttccct
      661 ttcttccttg tttctttttc tgcacaatat ttcaagctat accaagcata caatcaactc
      721 cggtaccact agtatggtaa gtaagggtga agaagacaat atggcgatca ttaaggaatt
      781 catgcgtttc aaagtacaca tggagggaag cgtgaacgga catgaatttg aaatcgaagg
      841 ggaaggcgaa ggtagaccat acgaaggaac ccagaccgca aagcttaaag ttaccaaagg
      901 cgggccacta ccatttgcat gggatatctt gagccctcag tttatgtatg gcagtaaggc
      961 ctacgttaaa cacccagctg atattcccga ctatttgaaa ttgtcttttc cagaaggatt
     1021 caaatgggaa agagtaatga atttcgagga cggcggagtt gttactgtta ctcaagattc
     1081 aagtttgcaa gacggtgaat ttatttacaa ggtcaaatta agagggacta atttccctag
     1141 tgatggtccc gtcatgcaaa agaagactat gggttgggaa gcctcatctg aacgtatgta
     1201 tccagaagat ggcgcgctta agggggaaat taaacaaaga ttgaagttaa aagacggtgg
     1261 tcactacgac gcggaagtta agaccactta taaagctaaa aagcccgttc agttacctgg
     1321 tgcatataac gtaaacatta aattggatat cacttcacat aatgaagatt acactattgt
     1381 ggaacaatat gaaagagctg aaggtaggca ctcaacgggt ggaatggacg aattgtacaa
     1441 ataaccgccg catagcttca aaatgtttct actccttttt tactcttcca gattttctcg
     1501 gactccgcgc atcgccgtac cacttcaaaa cacccaagca cagcatacta aatttcccct
     1561 ctttcttcct ctagggtgtc gttaattacc cgtactaaag gtttggaaaa gaaaaaagag
     1621 accgcctcgt ttctttttct tcgccgaaaa aggcgataaa aatttttatc acgtttcttt
     1681 ttcttgaaaa tttttttttt tgattttctt ctctttcgat gacctcccat tgatatttaa
     1741 gttaataaac ggtcttcaat ttctcaagtt tcagtttcat ttttcttgct ctattacaac
     1801 tttttttact tcttgctcat tagaaagaaa gcatagcaat ctaatctaag ttttccgcgg
     1861 ggtaccatgt ctaaaggtga agaattattc actggtgttg tcccaatttt ggttgaatta
     1921 gatggtgatg ttaatggtca caaattttct gtctccggtg aaggtgaagg tgatgctact
     1981 tacggtaaat tgaccttaaa atttatttgt actactggta aattgccagt tccatggcca
     2041 accttagtca ctactttaac ttatggtgtt caatgttttt ctagataccc agatcatatg
     2101 aaacaacatg actttttcaa gtctgccatg ccagaaggtt atgttcaaga aagaactatt
     2161 tttttcaaag atgacggtaa ctacaagacc agagctgaag tcaagtttga aggtgatacc
     2221 ttagttaata gaatcgaatt aaaaggtatt gattttaaag aagatggtaa cattttaggt
     2281 cacaaattgg aatacaacta taactctcac aatgtttaca tcatggctga caaacaaaag
     2341 aatggtatca aagttaactt caaaattaga cacaacattg aagatggttc tgttcaatta
     2401 gctgaccatt atcaacaaaa tactccaatt ggtgatggtc cagtcttgtt accagacaac
     2461 cattacttat ccactcaatc tgccttatcc aaagatccaa acgaaaagag agaccacatg
     2521 gtcttgttag aatttgttac tgctgctggt attacccatg gtatggatga attgtacaaa
     2581 gagatctcaa tcagtctgat tgcggcgtta gcggtagatc acgttatcgg catggaaaac
     2641 gccatgccgt ggaacctgcc tgccgatctc gcctggttta aacgcaacac cttaaataaa
     2701 cccgtgatta tgggccgcca tacctgggaa tcaatcggtc gtccgttgcc aggacgcaaa
     2761 aatattatcc tcagcagtca accgagtacg gacgatcgcg taacgtgggt gaagtcggtg
     2821 gatgaagcca tcgcggcgtg tggtgacgta ccagaaatca tggtgattgg cggcggtcgc
     2881 gtttatgaac agttcttgcc aaaagcgcaa aaactgtatc tgacgcatat cgacgcagaa
     2941 gtggaaggcg acacccattt cccggattac gagccggatg actgggaatc ggtattcagc
     3001 gaattccacg atgctgatgc gcagaactct cacagctatt gctttgagat tctggagcgg
     3061 cgataactcg agctgcagat gaatcgtaga tactgaaaaa ccccgcaagt tcacttcaac
     3121 tgtgcatcgt gcaccatctc aatttctttc atttatacat cgttttgcct tcttttatgt
     3181 aactatactc ctctaagttt caatcttggc catgtaacct ctgatctata gaatttttta
     3241 aatgactaga attaatgccc atcttttttt tggacctaaa ttcttcatga aaatatatta
     3301 cgagggctta ttcagaagct ttggacttct tcgccagagg tttggtcaag tctccaatca
     3361 aggttgtcgg cttgtctacc ttgccagaaa tttacgaaaa gatggaaaag ggtcaaatcg
     3421 ttggtagata cgttgttgac acttctaaat aagcgaattt cttatgattt atgattttta
     3481 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     3541 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     3601 tatagcatga ggtcgctctt attgaccaca cctctaccgg ccggtcgaaa ttcccctacc
     3661 ctatgaacat attccatttt gtaatttcgt gtcgtttcta ttatgaattt catttataaa
     3721 gtttatgtac aaatatcata aaaaaagaga atctttttaa gcaaggattt tcttaacttc
     3781 ttcggcgaca gcatcaccga cttcggtggt actgttggaa ccacctaaat caccagttct
     3841 gatacctgca tccaaaacct ttttaactgc atcttcaatg gccttacctt cttcaggcaa
     3901 gttcaatgac aatttcaaca tcattgcagc agacaagata gtggcgatag ggttgacctt
     3961 attctttggc aaatctggag cagaaccgtg gcatggttcg tacaaaccaa atgcggtgtt
     4021 cttgtctggc aaagaggcca aggacgcaga tggcaacaaa cccaaggaac ctgggataac
     4081 ggaggcttca tcggagatga tatcaccaaa catgttgctg gtgattataa taccatttag
     4141 gtgggttggg ttcttaacta ggatcatggc ggcagaatca atcaattgat gttgaacctt
     4201 caatgtagga aattcgttct tgatggtttc ctccacagtt tttctccata atcttgaaga
     4261 ggccaaaaca ttagctttat ccaaggacca aataggcaat ggtggctcat gttgtagggc
     4321 catgaaagcg gccattcttg tgattctttg cacttctgga acggtgtatt gttcactatc
     4381 ccaagcgaca ccatcaccat cgtcttcctt tctcttacca aagtaaatac ctcccactaa
     4441 ttctctgaca acaacgaagt cagtaccttt agcaaattgt ggcttgattg gagataagtc
     4501 taaaagagag tcggatgcaa agttacatgg tcttaagttg gcgtacaatt gaagttcttt
     4561 acggattttt agtaaacctt gttcaggtct aacactacct gtaccccatt taggaccacc
     4621 cacagcacct aacaaaacgg catcaacctt cttggaggct tccagcgcct catctggaag
     4681 tgggacacct gtagcgtcga tagcagcacc accaattaaa tgattttcga aatcgaactt
     4741 gacattggaa cgaacatcag aaatagcttt aagaacctta atggcttcgg ctgtgatttc
     4801 ttgaccaacg tggtcacctg gcaaaacgac gatcttctta ggggcagaca ttagaatggt
     4861 atatccttga aatatatata tatattgctg aaatgtaaaa ggtaagaaaa gttagaaagt
     4921 aagacgattg ctaaccacct attggaaaaa acaataggtc cttaaataat attgtcaact
     4981 tcaagtattg tgatgcaagc atttagtcat gaacgcttct ctattctata tgaaaagccg
     5041 gttccggcgc tctcaccttt cctttttctc ccaatttttc agttgaaaaa ggtatatgcg
     5101 tcaggcgacc tctgaaatta acaaaaaatt tccagtcatc gaatttgatt ctgtgcgata
     5161 gcgcccctgt gtgttctcgt tatgttgagg aaaaaaataa tggttgctaa gagattcgaa
     5221 ctcttgcatc ttacgatacc tgagtattcc cacagttggg gatctcgact ctagctagag
     5281 gatcaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5341 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     5401 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     5461 ccagctgata acttcgtata atgtatgcta tacgaagtta ttaggtctga agaggagttt
     5521 acgtccagcc aagctagctt ggctgcaggt cgagcggccg cgatccggaa cccttaatat
     5581 aacttcgtat aatgtatgct atacgaagtt atcagctgca ttaatgaatc ggccaacgcg
     5641 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     5701 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5761 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5821 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5881 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5941 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6001 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6061 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6121 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6181 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6241 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     6301 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6361 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6421 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6481 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6541 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6601 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6661 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6721 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6781 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6841 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6901 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     6961 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7021 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7081 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7141 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7201 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7261 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7321 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7381 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     7441 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     7501 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7561 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     7621 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     7681 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     7741 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     7801 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccataacg
     7861 catttaagca taaacacgca ctatgccgtt cttctcatgt atatatatat acaggcaaca
     7921 cgcagatata ggtgcgacgt gaacagtgag ctgtatgtgc gcagctcgcg ttgcattttc
     7981 ggaagcgctc gttttcggaa acgctttgaa gttcctattc cgaagttcct attctctagc
     8041 tagaaagtat aggaacttca gagcgctttt gaaaaccaaa agcgctctga agacgcactt
     8101 tcaaaaaacc aaaaacgcac cggactgtaa cgagctacta aaatattgcg aataccgctt
     8161 ccacaaacat tgctcaaaag tatctctttg ctatatatct ctgtgctata tccctatata
     8221 acctacccat ccacctttcg ctccttgaac ttgcatctaa actcgacctc tacatttttt
     8281 atgtttatct ctagtattac tctttagaca aaaaaattgt agtaagaact attcatagag
     8341 tgaatcgaaa acaatacgaa aatgtaaaca tttcctatac gtagtatata gagacaaaat
     8401 agaagaaacc gttcataatt ttctgaccaa tgaagaatca tcaacgctat cactttctgt
     8461 tcacaaagta tgcgcaatcc acatcggtat agaatataat cggggatgcc tttatcttga
     8521 aaaaatgcac ccgcagcttc gctagtaatc agtaaacgcg ggaagtggag tcaggctttt
     8581 tttatggaag agaaaataga caccaaagta gccttcttct aaccttaacg gacctacagt
     8641 gcaaaaagtt atcaagagac tgcattatag agcgcacaaa ggagaaaaaa agtaatctaa
     8701 gatgctttgt tagaaaaata gcgctctcgg gatgcatttt tgtagaacaa aaaagaagta
     8761 tagattcttt gttggtaaaa tagcgctctc gcgttgcatt tctgttctgt aaaaatgcag
     8821 ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc atttttgttt tacaaaaatg
     8881 aagcacagat tcttcgttgg taaaatagcg ctttcgcgtt gcatttctgt tctgtaaaaa
     8941 tgcagctcag attctttgtt tgaaaaatta gcgctctcgc gttgcatttt tgttctacaa
     9001 aatgaagcac agatgcttcg ttgct
//
