LOCUS       sequence-114595-        9679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes accA, accB, accC, accD in monocistronic configuration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 9679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pETM6-M.accABCD
FEATURES             Location/Qualifiers
     source          1..9679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1044..1088
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1141..1188
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1222..1240
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1241..1265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1280..1302
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1793..1837
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1890..1937
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1971..1989
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1990..2014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2029..2051
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3421..3465
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      3518..3565
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3599..3617
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3618..3642
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3657..3679
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4614..4658
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      4711..4758
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4802..5257
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5099..5120
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(5372..6163,6164..6232))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5995..6014
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6233..6325)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6406..6994
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6895..6914
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7148..7165
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7180..7322
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7248..7267)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7408..7430
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7424..7615)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8187..9269)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9231..9250
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9270..9347)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9553..9572
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(9663..9679,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgagtctg aatttccttg attttgaaca gccgattgca gagctggaag
      121 cgaaaatcga ttctctgact gcggttagcc gtcaggatga gaaactggat attaacatcg
      181 atgaagaagt gcatcgtctg cgtgaaaaaa gcgtagaact gacacgtaaa atcttcgccg
      241 atctcggtgc atggcagatt gcgcaactgg cacgccatcc acagcgtcct tataccctgg
      301 attacgttcg cctggcattt gatgaatttg acgaactggc tggcgaccgc gcgtatgcag
      361 acgataaagc tatcgtcggt ggtatcgccc gtctcgatgg tcgtccggtg atgatcattg
      421 gtcatcaaaa aggtcgtgaa accaaagaaa aaattcgccg taactttggt atgccagcgc
      481 cagaaggtta ccgcaaagca ctgcgtctga tgcaaatggc tgaacgcttt aagatgccta
      541 tcatcacctt tatcgacacc ccgggggctt atcctggcgt gggcgcagaa gagcgtggtc
      601 agtctgaagc cattgcacgc aacctgcgtg aaatgtctcg cctcggcgta ccggtagttt
      661 gtacggttat cggtgaaggt ggttctggcg gtgcgctggc gattggcgtg ggcgataaag
      721 tgaatatgct gcaatacagc acctattccg ttatctcgcc ggaaggttgt gcgtccattc
      781 tgtggaagag cgccgacaaa gcgccgctgg cggctgaagc gatgggtatc attgctccgc
      841 gtctgaaaga actgaaactg atcgactcca tcatcccgga accactgggt ggtgctcacc
      901 gtaacccgga agcgatggcg gcatcgttga aagcgcaact gctggcggat ctggccgatc
      961 tcgacgtgtt aagcactgaa gatttaaaaa atcgtcgtta tcagcgcctg atgagctacg
     1021 gttacgcgta actcgagtct ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca
     1081 tggactcgtc tactagtcgc agcttaatta acctaaactg ctgccaccgc tgagcaataa
     1141 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct aggatcgaga
     1201 tcgatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca
     1261 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tggatattcg
     1321 taagattaaa aaactgatcg agctggttga agaatcaggc atctccgaac tggaaatttc
     1381 tgaaggcgaa gagtcagtac gcattagccg tgcagctcct gccgcaagtt tccctgtgat
     1441 gcaacaagct tacgctgcac caatgatgca gcagccagct caatctaacg cagccgctcc
     1501 ggcgaccgtt ccttccatgg aagcgccagc agcagcggaa atcagtggtc acatcgtacg
     1561 ttccccgatg gttggtactt tctaccgcac cccaagcccg gacgcaaaag cgttcatcga
     1621 agtgggtcag aaagtcaacg tgggcgatac cctgtgcatc gttgaagcca tgaaaatgat
     1681 gaaccagatc gaagcggaca aatccggtac cgtgaaagca attctggtcg aaagtggaca
     1741 accggtagaa tttgacgagc cgctggtcgt catcgagtaa ctcgagtctg gtaaagaaac
     1801 cgctgctgcg aaatttgaac gccagcacat ggactcgtct actagtcgca gcttaattaa
     1861 cctaaactgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     1921 tcttgagggg ttttttgcta ggatcgagat cgatctcgat cccgcgaaat taatacgact
     1981 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
     2041 taagaaggag atatacatat gctggataaa attgttattg ccaaccgcgg cgagattgca
     2101 ttgcgtattc ttcgtgcctg taaagaactg ggcatcaaga ctgtcgctgt gcactccagc
     2161 gcggatcgcg atctaaaaca cgtattactg gcagatgaaa cggtctgtat tggccctgct
     2221 ccgtcagtaa aaagttatct gaacatcccg gcaatcatca gcgccgctga aatcaccggc
     2281 gcagtagcaa tccatccggg ttacggcttc ctctccgaga acgccaactt tgccgagcag
     2341 gttgaacgct ccggctttat cttcattggc ccgaaagcag aaaccattcg cctgatgggc
     2401 gacaaagtat ccgcaatcgc ggcgatgaaa aaagcgggcg tcccttgcgt accgggttct
     2461 gacggcccgc tgggcgacga tatggataaa aaccgtgcca ttgctaaacg cattggttat
     2521 ccggtgatta tcaaagcctc cggcggcggc ggcggtcgcg gtatgcgcgt agtgcgcggc
     2581 gacgctgaac tggcacaatc catctccatg acccgtgcgg aagcgaaagc tgctttcagc
     2641 aacgatatgg tttacatgga gaaatacctg gaaaatcctc gccacgtcga gattcaggta
     2701 ctggctgacg gtcagggcaa cgctatctat ctggcggaac gtgactgctc catgcaacgc
     2761 cgccaccaga aagtggtcga agaagcgcca gcaccgggca ttaccccgga actgcgtcgc
     2821 tacatcggcg aacgttgcgc taaagcgtgt gttgatatcg gctatcgcgg tgcaggtact
     2881 ttcgagttcc tgttcgaaaa cggcgagttc tatttcatcg aaatgaacac ccgtattcag
     2941 gtagaacacc cggttacaga aatgatcacc ggcgttgacc tgatcaaaga acagctgcgt
     3001 atcgctgccg gtcaaccgct gtcgatcaag caagaagaag ttcacgttcg cggccatgcg
     3061 gtggaatgtc gtatcaacgc cgaagatccg aacaccttcc tgccaagtcc gggcaaaatc
     3121 acccgtttcc acgcacctgg cggttttggc gtacgttggg agtctcatat ctacgcgggc
     3181 tacaccgtac cgccgtacta tgactcaatg atcggtaagc tgatttgcta cggtgaaaac
     3241 cgtgacgtgg cgattgcccg catgaagaat gcgctgcagg agctgatcat cgacggtatc
     3301 aaaaccaacg ttgatctgca gatccgcatc atgaatgacg agaacttcca gcatggtggc
     3361 actaacatcc actatctgga gaaaaaactc ggtcttcagg aaaaataact cgagtctggt
     3421 aaagaaaccg ctgctgcgaa atttgaacgc cagcacatgg actcgtctac tagtcgcagc
     3481 ttaattaacc taaactgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
     3541 taaacgggtc ttgaggggtt ttttgctagg atcgagatcg atctcgatcc cgcgaaatta
     3601 atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg
     3661 tttaacttta agaaggagat atacatatga gctggattga acgaattaaa agcaacatta
     3721 ctcccacccg caaggcgagc attcctgaag gggtgtggac taagtgtgat agctgcggtc
     3781 aggttttata ccgcgctgag ctggaacgta atcttgaggt ctgtccgaag tgtgaccatc
     3841 acatgcgtat gacagcgcgt aatcgcctgc atagcctgtt agatgaagga agccttgtgg
     3901 agctgggtag cgagcttgag ccgaaagatg tgctgaagtt tcgtgactcc aagaagtata
     3961 aagaccgtct ggcatctgcg cagaaagaaa ccggcgaaaa agatgcgctg gtggtgatga
     4021 aaggcactct gtatggaatg ccggttgtcg ctgcggcatt cgagttcgcc tttatgggcg
     4081 gttcaatggg gtctgttgtg ggtgcacgtt tcgtgcgtgc cgttgagcag gcgctggaag
     4141 ataactgccc gctgatctgc ttctccgcct ctggtggcgc acgtatgcag gaagcactga
     4201 tgtcgctgat gcagatggcg aaaacctctg cggcactggc aaaaatgcag gagcgcggct
     4261 tgccgtacat ctccgtgctg accgacccga cgatgggcgg tgtttctgca agtttcgcca
     4321 tgctgggcga tctcaacatc gctgaaccga aagcgttaat cggctttgcc ggtccgcgtg
     4381 ttatcgaaca gaccgttcgc gaaaaactgc cgcctggatt ccagcgcagt gaattcctga
     4441 tcgagaaagg cgcgatcgac atgatcgtcc gtcgtccgga aatgcgcctg aaactggcga
     4501 gcattctggc gaagttgatg aatctgccag cgccgaatcc tgaagcgccg cgtgaaggcg
     4561 tagtggtacc cccggtaccg gatcaggaac ctgaggcctg actcgagtct ggtaaagaaa
     4621 ccgctgctgc gaaatttgaa cgccagcaca tggactcgtc tactagtcgc agcttaatta
     4681 acctaaactg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     4741 gtcttgaggg gttttttgct agcgaaagga ggagtcgact atatccggat tggcgaatgg
     4801 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4861 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4921 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4981 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     5041 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5101 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     5161 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5221 aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat ggcatgagat
     5281 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5341 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5401 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5461 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5521 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5581 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5641 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5701 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5761 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5821 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5881 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5941 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6001 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6061 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6121 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6181 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6241 tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6301 gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac gtgagttttc
     6361 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6421 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6481 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6541 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6601 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6661 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6721 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6781 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6841 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6901 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6961 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7021 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7081 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     7141 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     7201 tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct
     7261 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     7321 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     7381 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     7441 catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt
     7501 cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg
     7561 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg
     7621 cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga
     7681 tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta
     7741 aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc
     7801 gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga
     7861 tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga
     7921 aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc
     7981 acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta
     8041 gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc caccggaagg
     8101 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga
     8161 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8221 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc
     8281 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg
     8341 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt
     8401 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact
     8461 accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc
     8521 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc
     8581 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga
     8641 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa
     8701 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg
     8761 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag
     8821 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg
     8881 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc
     8941 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc
     9001 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga
     9061 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg
     9121 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa
     9181 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct
     9241 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg
     9301 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg
     9361 ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag
     9421 caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac
     9481 ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg
     9541 atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt
     9601 gatgccggcc acgatgcgtc cggcgtagcc taggatcgag atcgatctcg atcccgcgaa
     9661 attaatacga ctcactata
//
