LOCUS       sequence-114597-         509 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry fused between Cry2 and gamma 9 to study cell migration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 509)
  AUTHORS   O'Neill PR, Gautam N
  TITLE     Subcellular optogenetic inhibition of G proteins generates signaling
            gradients and cell migration.
  JOURNAL   Mol Biol Cell. 2014 Aug 1;25(15):2305-14. doi: 
            10.1091/mbc.E14-04-0870. Epub 2014 Jun 11.
  PUBMED    24920824
REFERENCE   2  (bases 1 to 509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-04-0870"; journalName: "Mol Biol Cell"; date: 
            "2014-08-1- 1"; volume: "25"; issue: "15"; pages: "2305-14"
COMMENT     Sequence Label: Cry2 mcherry Gamma 9 in pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..509
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(346..363)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    352..463
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gnagggcgca ctccaccggc ggcatggacg agctgtacaa gggtaccatg catcaccatc
       61 accatcacgc ccaggatctc agcgagaagg acctgttgaa gatggaggtg gagcagctga
      121 agaaagaagt gaaaaacaca agaattccga tttccaaagc gggaaaggaa atcaaggagt
      181 acgtggaggc ccaagcagga aacgatcctt ttctcaaagg catccctgag gacaagaatc
      241 ccttcaagga aaaaggcggt tgtataataa gctgagaatt ctgcagatat ccagcacagt
      301 ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
      361 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
      421 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
      481 gtcattctat tcgggggggt ggggtgggg
//
