LOCUS       sequence-114599-        9391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes accA, accB, accC, accD in Pseudo-operon configuration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9391)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 9391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pETM6-P.accABCD
FEATURES             Location/Qualifiers
     source          1..9391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1044..1088
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        1126..1144
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1145..1169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1184..1206
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1697..1741
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        1779..1797
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1798..1822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1837..1859
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3229..3273
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        3311..3329
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3330..3354
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3369..3391
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4326..4370
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(4409..4427)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4423..4470
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4514..4969
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4811..4832
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(5084..5875,5876..5944))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5707..5726
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5945..6037)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6118..6706
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6607..6626
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6860..6877
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6892..7034
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6960..6979)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7120..7142
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7136..7327)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7899..8981)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8943..8962
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8982..9059)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9265..9284
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(9375..9391,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgagtctg aatttccttg attttgaaca gccgattgca gagctggaag
      121 cgaaaatcga ttctctgact gcggttagcc gtcaggatga gaaactggat attaacatcg
      181 atgaagaagt gcatcgtctg cgtgaaaaaa gcgtagaact gacacgtaaa atcttcgccg
      241 atctcggtgc atggcagatt gcgcaactgg cacgccatcc acagcgtcct tataccctgg
      301 attacgttcg cctggcattt gatgaatttg acgaactggc tggcgaccgc gcgtatgcag
      361 acgataaagc tatcgtcggt ggtatcgccc gtctcgatgg tcgtccggtg atgatcattg
      421 gtcatcaaaa aggtcgtgaa accaaagaaa aaattcgccg taactttggt atgccagcgc
      481 cagaaggtta ccgcaaagca ctgcgtctga tgcaaatggc tgaacgcttt aagatgccta
      541 tcatcacctt tatcgacacc ccgggggctt atcctggcgt gggcgcagaa gagcgtggtc
      601 agtctgaagc cattgcacgc aacctgcgtg aaatgtctcg cctcggcgta ccggtagttt
      661 gtacggttat cggtgaaggt ggttctggcg gtgcgctggc gattggcgtg ggcgataaag
      721 tgaatatgct gcaatacagc acctattccg ttatctcgcc ggaaggttgt gcgtccattc
      781 tgtggaagag cgccgacaaa gcgccgctgg cggctgaagc gatgggtatc attgctccgc
      841 gtctgaaaga actgaaactg atcgactcca tcatcccgga accactgggt ggtgctcacc
      901 gtaacccgga agcgatggcg gcatcgttga aagcgcaact gctggcggat ctggccgatc
      961 tcgacgtgtt aagcactgaa gatttaaaaa atcgtcgtta tcagcgcctg atgagctacg
     1021 gttacgcgta actcgagtct ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca
     1081 tggactcgtc tactaggatc gagatcgatc tcgatcccgc gaaattaata cgactcacta
     1141 taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga
     1201 aggagatata catatggata ttcgtaagat taaaaaactg atcgagctgg ttgaagaatc
     1261 aggcatctcc gaactggaaa tttctgaagg cgaagagtca gtacgcatta gccgtgcagc
     1321 tcctgccgca agtttccctg tgatgcaaca agcttacgct gcaccaatga tgcagcagcc
     1381 agctcaatct aacgcagccg ctccggcgac cgttccttcc atggaagcgc cagcagcagc
     1441 ggaaatcagt ggtcacatcg tacgttcccc gatggttggt actttctacc gcaccccaag
     1501 cccggacgca aaagcgttca tcgaagtggg tcagaaagtc aacgtgggcg ataccctgtg
     1561 catcgttgaa gccatgaaaa tgatgaacca gatcgaagcg gacaaatccg gtaccgtgaa
     1621 agcaattctg gtcgaaagtg gacaaccggt agaatttgac gagccgctgg tcgtcatcga
     1681 gtaactcgag tctggtaaag aaaccgctgc tgcgaaattt gaacgccagc acatggactc
     1741 gtctactagg atcgagatcg atctcgatcc cgcgaaatta atacgactca ctatagggga
     1801 attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta agaaggagat
     1861 atacatatgc tggataaaat tgttattgcc aaccgcggcg agattgcatt gcgtattctt
     1921 cgtgcctgta aagaactggg catcaagact gtcgctgtgc actccagcgc ggatcgcgat
     1981 ctaaaacacg tattactggc agatgaaacg gtctgtattg gccctgctcc gtcagtaaaa
     2041 agttatctga acatcccggc aatcatcagc gccgctgaaa tcaccggcgc agtagcaatc
     2101 catccgggtt acggcttcct ctccgagaac gccaactttg ccgagcaggt tgaacgctcc
     2161 ggctttatct tcattggccc gaaagcagaa accattcgcc tgatgggcga caaagtatcc
     2221 gcaatcgcgg cgatgaaaaa agcgggcgtc ccttgcgtac cgggttctga cggcccgctg
     2281 ggcgacgata tggataaaaa ccgtgccatt gctaaacgca ttggttatcc ggtgattatc
     2341 aaagcctccg gcggcggcgg cggtcgcggt atgcgcgtag tgcgcggcga cgctgaactg
     2401 gcacaatcca tctccatgac ccgtgcggaa gcgaaagctg ctttcagcaa cgatatggtt
     2461 tacatggaga aatacctgga aaatcctcgc cacgtcgaga ttcaggtact ggctgacggt
     2521 cagggcaacg ctatctatct ggcggaacgt gactgctcca tgcaacgccg ccaccagaaa
     2581 gtggtcgaag aagcgccagc accgggcatt accccggaac tgcgtcgcta catcggcgaa
     2641 cgttgcgcta aagcgtgtgt tgatatcggc tatcgcggtg caggtacttt cgagttcctg
     2701 ttcgaaaacg gcgagttcta tttcatcgaa atgaacaccc gtattcaggt agaacacccg
     2761 gttacagaaa tgatcaccgg cgttgacctg atcaaagaac agctgcgtat cgctgccggt
     2821 caaccgctgt cgatcaagca agaagaagtt cacgttcgcg gccatgcggt ggaatgtcgt
     2881 atcaacgccg aagatccgaa caccttcctg ccaagtccgg gcaaaatcac ccgtttccac
     2941 gcacctggcg gttttggcgt acgttgggag tctcatatct acgcgggcta caccgtaccg
     3001 ccgtactatg actcaatgat cggtaagctg atttgctacg gtgaaaaccg tgacgtggcg
     3061 attgcccgca tgaagaatgc gctgcaggag ctgatcatcg acggtatcaa aaccaacgtt
     3121 gatctgcaga tccgcatcat gaatgacgag aacttccagc atggtggcac taacatccac
     3181 tatctggaga aaaaactcgg tcttcaggaa aaataactcg agtctggtaa agaaaccgct
     3241 gctgcgaaat ttgaacgcca gcacatggac tcgtctacta ggatcgagat cgatctcgat
     3301 cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta
     3361 gaaataattt tgtttaactt taagaaggag atatacatat gagctggatt gaacgaatta
     3421 aaagcaacat tactcccacc cgcaaggcga gcattcctga aggggtgtgg actaagtgtg
     3481 atagctgcgg tcaggtttta taccgcgctg agctggaacg taatcttgag gtctgtccga
     3541 agtgtgacca tcacatgcgt atgacagcgc gtaatcgcct gcatagcctg ttagatgaag
     3601 gaagccttgt ggagctgggt agcgagcttg agccgaaaga tgtgctgaag tttcgtgact
     3661 ccaagaagta taaagaccgt ctggcatctg cgcagaaaga aaccggcgaa aaagatgcgc
     3721 tggtggtgat gaaaggcact ctgtatggaa tgccggttgt cgctgcggca ttcgagttcg
     3781 cctttatggg cggttcaatg gggtctgttg tgggtgcacg tttcgtgcgt gccgttgagc
     3841 aggcgctgga agataactgc ccgctgatct gcttctccgc ctctggtggc gcacgtatgc
     3901 aggaagcact gatgtcgctg atgcagatgg cgaaaacctc tgcggcactg gcaaaaatgc
     3961 aggagcgcgg cttgccgtac atctccgtgc tgaccgaccc gacgatgggc ggtgtttctg
     4021 caagtttcgc catgctgggc gatctcaaca tcgctgaacc gaaagcgtta atcggctttg
     4081 ccggtccgcg tgttatcgaa cagaccgttc gcgaaaaact gccgcctgga ttccagcgca
     4141 gtgaattcct gatcgagaaa ggcgcgatcg acatgatcgt ccgtcgtccg gaaatgcgcc
     4201 tgaaactggc gagcattctg gcgaagttga tgaatctgcc agcgccgaat cctgaagcgc
     4261 cgcgtgaagg cgtagtggta cccccggtac cggatcagga acctgaggcc tgactcgagt
     4321 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagtc
     4381 gcagcttaat taacctaaac tgctgccacc gctgagcaat aactagcata accccttggg
     4441 gcctctaaac gggtcttgag gggttttttg ctagcgaaag gaggagtcga ctatatccgg
     4501 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4561 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4621 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4681 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4741 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4801 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4861 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4921 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc tggcggcacg
     4981 atggcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     5041 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5101 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5161 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5221 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     5281 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     5341 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     5401 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     5461 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     5521 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     5581 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     5641 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     5701 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     5761 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     5821 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5881 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5941 tcatactctt cctttttcaa tcatgattga agcatttatc agggttattg tctcatgagc
     6001 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gtcatgacca aaatccctta
     6061 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6121 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6181 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6241 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     6301 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6361 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6421 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6481 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6541 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6601 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6661 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6721 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6781 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6841 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     6901 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt
     6961 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     7021 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     7081 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     7141 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     7201 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     7261 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     7321 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     7381 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     7441 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     7501 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     7561 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     7621 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     7681 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     7741 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgctagtc atgccccgcg
     7801 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg
     7861 cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7921 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7981 gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc
     8041 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg
     8101 cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg
     8161 tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt aatggcgcgc
     8221 attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca
     8281 ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc
     8341 gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc
     8401 gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc
     8461 agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt
     8521 gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca
     8581 atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga
     8641 agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc
     8701 acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg
     8761 tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt
     8821 tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc
     8881 gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca
     8941 ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga
     9001 ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc
     9061 gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
     9121 gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag
     9181 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
     9241 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
     9301 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag cctaggatcg agatcgatct
     9361 cgatcccgcg aaattaatac gactcactat a
//
