LOCUS       sequence-114603-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 
            528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: miniSOG-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..352
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
ORIGIN
        1 ccgtcagaat ccgctagcgc taccggtcgc caccatggag aaaagtttcg tgataactga
       61 tccacggctg ccagacaatc ccatcatctt cgcatccgat ggcttcctgg agctgaccga
      121 gtattccaga gaggagatcc tgggccgcaa tggccgcttt ctgcagggac cagagacaga
      181 ccaggccaca gtgcagaaga ttcgcgatgc cattagagat cagcgcgaga ttaccgtgca
      241 gctgataaac tacacaaaaa gcgggaagaa attctggaac ctcctgcacc tccagcccat
      301 gagggaccag aagggtgagc tccagtattt catcggagtg cagctggatg gatccggact
      361 cagatctcga gtgcgtgagt gcatctccat ccacgttggc caggctggtg tccagattgg
      421 caatgcctgc tgggagctct actgcctgga acacggcatc cagcccgatg gccagatgcc
      481 aagtgacaag accattgggg gaggagatga ctccttcaac accttcttca gtgagacggg
      541 cgctggcaag cacgtgcccc gggctgtgtt tgtagacttg gaacccacag tcattgatga
      601 agttcgcact ggcacctacc gccagctctt ccaccctgag cagctcatca caggcaagga
      661 agatgctgcc aataactatg cccgagggca ctacaccatt ggcaaggaga tcattgacct
      721 tgtgttggac cgaattcgca agctggctga ccagtgcacc ggtcttcagg gcttcttggt
      781 tttccacagc tttggtgggg gaactggttc tgggttcacc tccctgctca tggaacgtct
      841 ctcagttgat tatggcaaga agtccaagct
//
