LOCUS       sequence-114607-         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: miniSOG-Actin-7
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..346
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="EKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQGP
                     ETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQLD
                     G"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg ggagaaaagt ttcgtgataa ctgatccacg
       61 gctgccagac aatcccatca tcttcgcatc cgatggcttc ctggagctga ccgagtattc
      121 cagagaggag atcctgggcc gcaatggccg ctttctgcag ggaccagaga cagaccaggc
      181 cacagtgcag aagattcgcg atgccattag agatcagcgc gagattaccg tgcagctgat
      241 aaactacaca aaaagcggga agaaattctg gaacctcctg cacctccagc ccatgaggga
      301 ccagaagggt gagctccagt atttcatcgg agtgcagctg gatggatccg gactcagatc
      361 tcgagctatg gatgatgata tcgccgcgct cgtcgtcgac aacggctccg gcatgtgcaa
      421 ggccggcttc gcgggcgacg atgccccccg ggccgtcttc ccctccatcg tggggcgccc
      481 caggcaccag ggcgtgatgg tgggcatggg tcagaaggat tcctatgtgg gcgacgaggc
      541 ccagagcaag agaggcatcc tcaccctgaa gtaccccatc gagcacggca tcgtcaccaa
      601 ctgggacgac atggagaaaa tctggcacca caccttctac aatgagctgc gtgtggctcc
      661 cgaggagcac cccgtgctgc tgaccgaggc ccccctgaac cccaaggcca ac
//
