LOCUS       sequence-114614-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 485, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: sfGFP-ER-3
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(175..885,886..888,889..891))
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(217..238)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     825..846
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
       61 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtta tctagatccg
      121 gtggatcccg ggcccgcggt accgtcgact gcagaattct tacagctcgt ccttcttgta
      181 cagctcgtcc atgccgtgag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      241 atcgcgcttc tcgttggggt ctttgctcag cacggactgg gtgctcaggt agtggttgtc
      301 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
      361 cacgctgccg tcctccacgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg
      421 cttgtcggcg gtgatataga cgttgtggct gttgaagttg tactccagct tgtgccccag
      481 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      541 gccctcgaac ttcacctcgg cgcgggtctt gtaggtgccg tcgtccttga agctgatggt
      601 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtggc gcttcatgtg
      661 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
      721 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgttggtggc
      781 atcgccctcg ccctcgccgc gcacgctgaa cttgtggccg tttacgtcgc cgtccagctc
      841 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tcgaccggtc
      901 ggcgacggcc a
//
