LOCUS       sequence-11462-e        9806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9806)
  TITLE     pLAI-Delta-mls
REFERENCE   2  (bases 1 to 9806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLAI-Delta-mls
FEATURES             Location/Qualifiers
     source          1..9806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..23
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7..23
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     LTR             54..687
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    734..859
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2938..3055
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5937..6170
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6355..6399
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             6693..6719
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     LTR             7254..7887
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7916..7934)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8002..8106
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8107..8175,8176..8967)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8325..8344)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9138..9726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9627..9646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaattctcta gaactagtgg atcttagcca ctttttaaaa gaaaaggggg gactggaagg
       61 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
      121 ctacttccct gattggcaga actacacacc agggccaggg gtcagatatc cactgacctt
      181 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
      241 agagaacacc agcttgttac accctgtgag cctgcatgga atggatgacc ctgagagaga
      301 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
      361 tccggagtac ttcaagaact gctgacatcg agcttgctac aagggacttt ccgctgggga
      421 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatgctgcat
      481 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatt tgagcctggg
      541 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      601 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      661 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      721 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      781 cgaggggagg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      841 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgatgg gaaaaaattc
      901 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
      961 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1021 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1081 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1141 ctttagacaa gatagaggaa gagcaaaaca aaagtaagaa aaaagcacag caagcagcag
     1201 ctgacacagg acacagcagc caggtcagcc aaaattaccc tatagtgcag aacatccagg
     1261 ggcaaatggt acatcaggcc atatcaccta gaactttaaa tgcatgggta aaagtagtag
     1321 aagagaaggc tttcagccca gaagtgatac ccatgttttc agcattatca gaaggagcca
     1381 ccccacaaga tttaaacacc atgctaaaca cagtgggggg acatcaagca gccatgcaaa
     1441 tgttaaaaga gaccatcaat gaggaagctg cagaatggga tagagtgcat ccagtgcatg
     1501 cagggcctat tgcaccaggc cagatgagag aaccaagggg aagtgacata gcaggaacta
     1561 ctagtaccct tcaggaacaa ataggatgga tgacaaataa tccacctatc ccagtaggag
     1621 aaatttataa aagatggata atcctgggat taaataaaat agtaagaatg tatagcccta
     1681 ccagcattct ggacataaga caaggaccaa aagaaccctt tagagactat gtagaccggt
     1741 tctataaaac tctaagagcc gagcaagctt cacaggaggt aaaaaattgg atgacagaaa
     1801 ccttgttggt ccaaaatgcg aacccagatt gtaagactat tttaaaagca ttgggaccag
     1861 cagctacact agaagaaatg atgacagcat gtcagggagt gggaggaccc ggccataagg
     1921 caagagtttt ggctgaagca atgagccaag taacaaattc agctaccata atgatgcaaa
     1981 gaggcaattt taggaaccaa agaaagattg ttaagtgttt caattgtggc aaagaagggc
     2041 acatagccag aaattgcagg gcccctagga aaaagggctg ttggaaatgt ggaaaggaag
     2101 gacaccaaat gaaagattgt actgagagac aggctaattt tttagggaag atctggcctt
     2161 cctacaaggg aaggccaggg aattttcttc agagcagacc agagccaaca gccccaccat
     2221 ttcttcagag cagaccagag ccaacagccc caccagaaga gagcttcagg tctggggtag
     2281 agacaacaac tccctctcag aagcaggagc cgatagacaa ggaactgtat cctttaactt
     2341 ccctcagatc actctttggc aacgacccct cgtcacaata aagatagggg ggcaactaaa
     2401 ggaagctcta ttagatacag gagcagatga tacagtatta gaagaaatga gtttgccagg
     2461 aagatggaaa ccaaaaatga tagggggaat tggaggtttt atcaaagtaa gacagtatga
     2521 tcagatactc atagaaatct gtggacataa agctataggt acagtattag taggacctac
     2581 acctgtcaac ataattggaa gaaatctgtt gactcagatt ggttgcactt taaattttcc
     2641 cattagtcct attgaaactg taccagtaaa attaaagcca ggaatggatg gcccaaaagt
     2701 taaacaatgg ccagtaaaaa caatacatac agacaatggc agcaatttca ccagtactac
     2761 ggttaaggcc gcctgttggt gggcgggaat caagcaggaa tttggaattc cctacaatcc
     2821 ccaaagtcaa ggagtagtag aatctatgaa taaagaatta aagaaaatta taggccaggt
     2881 aagagatcag gctgaacatc ttaagacagc agtacaaatg gcagtattca tccacaattt
     2941 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     3001 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     3061 ttattacagg gacagcagag atccactttg gaaaggacca gcaaagctcc tctggaaagg
     3121 tgaaggggca gtagtaatac aagataatag tgacataaaa gtagtgccaa gaagaaaagc
     3181 aaagatcatt agggattatg gaaaacagat ggcaggtgat gattgtgtgg caagtagaca
     3241 ggatgaggat tagaacatgg aaaagtttag taaaacacca tatgtatgtt tcagggaaag
     3301 ctaggggatg gttttataga catcactatg aaagccctca tccaagaata agttcagaag
     3361 tacacatccc actaggggat gctagattgg taataacaac atattggggt ctgcatacag
     3421 gagaaagaga ctggcatctg ggtcagggag tctccataga atggaggaaa aagagatata
     3481 gcacacaagt agaccctgaa ctagcagacc aactaattca tctgtattac tttgactgtt
     3541 tttcagactc tgctataaga aaggccttat taggacatat agttagccct aggtgtgaat
     3601 atcaagcagg acataacaag gtaggatctc tacaatactt ggcactagca gcattaataa
     3661 caccaaaaaa gataaagcca cctttgccta gtgttacgaa actgacagag gatagatgga
     3721 acaagcccca gaagaccaag ggccacagag ggagccacac aatgaatgga cactagagct
     3781 tttagaggag cttaagaatg aagctgttag acattttcct aggatttggc tccatggctt
     3841 agggcaacat atctatgaaa cttatgggga tacttgggca ggagtggaag ccataataag
     3901 aattctgcaa caactgctgt ttatccattt cagaattggg tgtcgacata gcagaatagg
     3961 cgttactcaa cagaggagag caagaaatgg agccagtaga tcctagacta gagccctgga
     4021 agcatccagg aagtcagcct aaaactgctt gtaccacttg ctattgtaaa aagtgttgct
     4081 ttcattgcca agtttgtttc acaacaaaag ccttaggcat ctcctatggc aggaagaagc
     4141 ggagacagcg acgaagacct cctcaaggca gtcagactca tcaagtttct ctatcaaagc
     4201 agtaagtagt acatgtaatg caacctatac aaatagcaat agcagcatta gtagtagcaa
     4261 taataatagc aatagttgtg tggtccatag taatcataga atataggaaa atattaagac
     4321 aaagaaaaat agacaggtta attgatagac taatagaaag agcagaagac agtggcaatg
     4381 agagtgaagg agaaatatca gcacttgtgg agatgggggt ggaaatgggg caccatgctc
     4441 cttgggatat tgatgatctg tagtgctaca gaaaaattgt gggtcacagt ctattatggg
     4501 gtacctgtgt ggaaggaagc aaccaccact ctattttgtg catcagatgc taaagcatat
     4561 gatacagagg tacataatgt ttgggccaca catgcctgtg tacccacaga ccccaaccca
     4621 caagaagtag tattggtaaa tgtgacagaa aattttaaca tgtggaaaaa tgacatggta
     4681 gaacagatgc atgaggatat aatcagttta tgggatcaaa gcctaaagcc atgtgtaaaa
     4741 ttaaccccac tctgtgttag tttaaagtgc actgatttgg ggaatgctac taataccaat
     4801 agtagtaata ccaatagtag tagcggggaa atgatgatgg agaaaggaga gataaaaaac
     4861 tgctctttca atatcagcac aagcataaga ggtaaggtgc agaaagaata tgcatttttt
     4921 tataaacttg atataatacc aatagataat gatactacca gctatacgtt gacaagttgt
     4981 aacacctcag tcattacaca ggcctgtcca aaggtatcct ttgagccaat tcccatacat
     5041 tattgtgccc cggctggttt tgcgattcta aaatgtaata ataagacgtt caatggaaca
     5101 ggaccatgta caaatgtcag cacagtacaa tgtacacatg gaattaggcc agtagtatca
     5161 actcaactgc tgttgaatgg cagtctagca gaagaagagg tagtaattag atctgccaat
     5221 ttcacagaca atgctaaaac cataatagta cagctgaacc aatctgtaga aattaattgt
     5281 acaagaccca acaacaatac aagaaaaagt atccgtatcc agaggggacc agggagagca
     5341 tttgttacaa taggaaaaat aggaaatatg agacaagcac attgtaacat tagtagagca
     5401 aaatggaatg ccactttaaa acagatagct agcaaattaa gagaacaatt tggaaataat
     5461 aaaacaataa tctttaagca atcctcagga ggggacccag aaattgtaac gcacagtttt
     5521 aattgtggag gggaattttt ctactgtaat tcaacacaac tgtttaatag tacttggttt
     5581 aatagtactt ggagtactga agggtcaaat aacactgaag gaagtgacac aatcacactc
     5641 ccatgcagaa taaaacaatt tataaacatg tggcaggaag taggaaaagc aatgtatgcc
     5701 cctcccatca gcggacaaat tagatgttca tcaaatatta cagggctgct attaacaaga
     5761 gatggtggta ataacaacaa tgggtccgag atcttcagac ctggaggagg agatatgagg
     5821 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     5881 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     5941 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcacg gtcaatgacg
     6001 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     6061 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     6121 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     6181 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     6241 aaatctctgg aacagatttg gaataacatg acctggatgg agtgggacag agaaattaac
     6301 aattacacaa gcttaataca ttccttaatt gaagaatcgc aaaaccagca agaaaagaat
     6361 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     6421 aattggctgt ggtatataaa aatattcata atgatagtag gaggcttggt aggtttaaga
     6481 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     6541 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     6601 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcctt agcacttatc
     6661 tgggacgatc tgcggagcct gtgcctcttc agctaccacc gcttgagaga cttactcttg
     6721 attgtaacga ggattgtgga acttctggga cgcagggggt gggaagccct caaatattgg
     6781 tggaatctcc tacagtattg gagtcaggaa ctaaagaata gtgctgttag cttgctcaat
     6841 gccacagcca tagcagtagc tgaggggaca gatagggtta tagaagtagt acaaggagct
     6901 tgtagagcta ttcgccacat acctagaaga ataagacagg gcttggaaag gattttgcta
     6961 taagatgggt ggcaagtggt caaaaagtag tgtggttgga tggcctactg taagggaaag
     7021 aatgagacga gctgagccag cagcagatgg ggtgggagca gcatctcgag acctggaaaa
     7081 acatggagca atcacaagta gcaatacagc agctaccaat gctgcttgtg cctggctaga
     7141 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     7201 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7261 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     7321 ctacttccct gattggcaga actacacacc agggccaggg gtcagatatc cactgacctt
     7381 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
     7441 agagaacacc agcttgttac accctgtgag cctgcatgga atggatgacc ctgagagaga
     7501 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
     7561 tccggagtac ttcaagaact gctgacatcg agcttgctac aagggacttt ccgctgggga
     7621 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatgctgcat
     7681 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatt tgagcctggg
     7741 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7801 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7861 tttagtcagt gtggaaaatc tctagcagtg gcgatgaatt cttgaagacg aaagggcctc
     7921 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     7981 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     8041 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     8101 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     8161 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     8221 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     8281 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     8341 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     8401 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     8461 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     8521 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     8581 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     8641 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     8701 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     8761 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     8821 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     8881 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     8941 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     9001 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     9061 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     9121 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     9181 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     9241 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     9301 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     9361 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     9421 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     9481 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     9541 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9601 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9661 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9721 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9781 cacatgttct ttcctgcgtt atccgc
//
