LOCUS       sequence-114622-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Nallamsetty S, Austin BP, Penrose KJ, Waugh DS
  TITLE     Gateway vectors for the production of combinatorially-tagged 
            His6-MBP fusion proteins in the cytoplasm and periplasm of 
            Escherichia coli.
  JOURNAL   Protein Sci. 2005 Dec . 14(12):2964-71.
  PUBMED    16322578
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2005 Dec . 14(12):2964-71."
COMMENT     Sequence Label: pDEST-HisMBP
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(353..358,359..374,375..381)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    389..405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     394..416
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             484..501
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
       61 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      121 attaatgtaa gttagctcac tcattaggca caattctcat gtttgacagc ttatcatcga
      181 ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg gtatggctgt
      241 gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt tctggataat
      301 gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat
      361 taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      421 agccagtccg tttaggtgtt ttcacgagca cttcaccaac aaggaccata gattatgaaa
      481 atccatcacc atcaccatca cgaagaaggt aaactggtaa tctggattaa cggcgataaa
      541 ggctataacg gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc
      601 accgttgagc atccggataa actggaagag aaattcccac aggttgcggc aactggcgat
      661 ggccctgaca ttatcttctg ggcacacgac cgctttggtg gctacgctca atctggcctg
      721 ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat
      781 gccgtacgtt acaacggcaa gctgattgct tacccgatcg ctgttgaagc gttatcg
//
