LOCUS       sequence-114623-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 400 / 490, Emission: 468 / 
            511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Mito-7
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..92
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             126..842
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 ccgtcagatc cgctagcatg tccgtcctga cgccgctgct gctgcggggc ttgacaggct
       61 cggcccggcg gctcccagtg ccgcgcgcca agatccattc gttgggggat ccaccggtcg
      121 ccaccatgag caagggcgcc gagctgttca ccggcatcgt gcccatcctg atcgagctga
      181 atggcgatgt gaatggccac aagttcagcg tgagcggcga gggcgagggc gatgccacct
      241 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcctgtg ccctggccca
      301 ccctggtggc caccctgagc tacggcgtgc agtgcttctc acgctacccc gatcacatga
      361 agcagcacga cttcttcaag agcgccatgc ctgagggcta catccaggag cgcaccatct
      421 tcttcgagga tgacggcaac tacaagacgc gcgccgaggt gaagttcgag ggcgataccc
      481 tggtgagtcg catcgagctg accggcactg atttcaagga ggatggcaac atcctgggca
      541 ataagatgga gtacaactac aacgccacca atgtgtacat cgtggccgac aaggccagga
      601 atggcatcaa ggtgaacttc aaggtccgcc acaacatcaa ggatggcagc gtgcagctgg
      661 ccgaccacta ccagcagaat acccccatcg gcgatggccc tgtgctgctg cccgataacc
      721 actacctgtc cacccagagc gccctgtcca aggaccccaa cgagaagcgc gatcacatga
      781 tctacctcga gttcgtgacc gccgccgcca tcacccacgg catggatgag ctgtacaagt
      841 aaagcggccg cgactctaga tcataatcag
//
