LOCUS       sequence-114652-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Clover-mRuby2 tandem fusion for FRET calibration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     Beam Lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1-Clover-mRuby2
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(65..775)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     CDS             complement(join(782..796,797..805))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 aatagggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat ctgcagaatt
       61 cttacttgta cagctcgtcc atcccaccac caagcccggc gaacttggca actgcgtgtt
      121 cgcgttgtac tacgaacatt tcattgtcac tttcctctaa cctttccagg cggtgatcaa
      181 cggcatggat accgggcatc ttgatgttcc cgacggtctt ttttgacctg taagttgtta
      241 cgaaagagca agacagatgg ccaccaccat caactttcag tgccatatga gtgtatcccc
      301 tcagaccacc atctgctgga tacatcatct ctgtattagg ctcccaaccc ttggtcttct
      361 tctgcatcac gggaccattg gagggaaagt ttacccctct gacttggacg tggtaaacga
      421 gacagccatc ctcaaggctg gtgtcctgca tgacggtgac gactccacca tcttcgtatc
      481 tcgtaactct ttcccaagta aaaccctcag gaaaggactg tttaaagaaa tcaggaatgc
      541 ctttcgggta cttgataaaa gtacggctgc catacatgaa cgacgtggca agaatgtcaa
      601 aggcaaatgg caggggtcct ccctcgatga ctttgatcct catggtttga gttcccatgt
      661 acggattgcc ttctccttca cctgtgcatt tgaattggtg gccgttgacc gaaccttcca
      721 tgaccacctt catacgcata ttttccttga tcagctcttc gcccttagac accatcggat
      781 ccttatcgtc atcgtcgtac agatcccgac ccatttgctg tccaccagtc atgctagact
      841 tgtacagctc gtccatgcca tgtgtaatcc cggcggcggt cacgaactcc a
//
