LOCUS       sequence-114666-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD S377R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS512 (TJF032 S377R)
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    78..102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             117..139
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg atctcgatcc cgcgaaatta
       61 atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg
      121 tttaacttta agaaggagat ataccatggc attgaagaag gtttggctta accgttatcc
      181 cgcggacgtt ccgacggaga tcaaccctga ccgttatcaa tctctggtag atatgtttga
      241 gcagtcggtc gcgcgctacg ccgatcaacc tgcgtttgtg aatatggggg aggtaatgac
      301 cttccgcaag ctggaagaac gcagtcgcgc gtttgccgct tatttgcaac aagggttggg
      361 gctgaagaaa ggcgatcgcg ttgcgttgat gatgcctaat ttattgcaat atccggtggc
      421 gctgtttggc attttgcgtg ccgggatgat cgtcgtaaac gttaacccgt tgtatacccc
      481 gcgtgagctt gagcatcagc ttaacgatag cggcgcatcg gcgattgtta tcgtgtctaa
      541 ctttgctcac acactggaaa aagtggttga taaaaccgcc gttcagcacg taattctgac
      601 ccgtatgggc gatcagctat ctacggcaaa aggcacggta gtcaatttcg ttgttaaata
      661 catcaagcgt ttggtgccga aataccatct gccagatgcc atttcatttc gtagcgcact
      721 gcataacggc taccggatgc agtacgtcaa acccgaactg gtgccggaag atttagcttt
      781 tctgcaatac accggcggca ccactggtgt ggcgaaaggc gcgatgctga ctcaccgcaa
      841 tatgctggcg aacctggaac aggttaacgc gacctatggt ccgctgttgc atccgggcaa
      901 agagctggtg gtgacggcgc tgccgctgta tcacattttt gccctgacca ttaactgcct
      961 gctgtttatc gaactggg
//
