LOCUS       sequence-114676-         208 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) 
            in a Cre-dependent manner in an AAV backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 208)
  AUTHORS   Chen Y, Saulnier JL, Yellen G, Sabatini BL
  TITLE     A PKA activity sensor for quantitative analysis of endogenous GPCR 
            signaling via 2-photon FRET-FLIM imaging.
  JOURNAL   Front Pharmacol. 2014 Apr 2;5:56. doi: 10.3389/fphar.2014.00056. 
            eCollection 2014.
  PUBMED    24765076
REFERENCE   2  (bases 1 to 208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Pharmacol."; date: "2014-04-2"; volume: "5:56. doi"; pages: " 
            10.3389/fphar.2014.00056. eCollection 2014"
COMMENT     Sequence Label: AAV-FLEX-PKIalpha-IRES-nls-mRuby2
FEATURES             Location/Qualifiers
     source          1..208
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcggcttcgg ccagtaacgt tagggggggg gggctagctt agctttcana cttggctgct
       61 tctccctggg cttctccact ttgttcggtg gagcttctct gtccatcatc ttcaccttct
      121 gtcttgttga tatcaaggcc tgctagtttt aaggctaatt cattgctgtt gccacttgca
      181 gaggaaacca ggatatcatg tattgcat
//
