LOCUS       sequence-114683-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD D372G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS506 (TJF069)
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    140..164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(164..183)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(165..183)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(272..289)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggcgttcaaa tttcgcagca gcggtttctt taccagactc gagggtaccg acgtcagcga
       61 tcgcgtggcc ggccgatatc caattgagat ctgccatatg tatatctcct tcttatactt
      121 aactaatata ctaagatggg gaattgttat ccgctcacaa ttcccctata gtgagtcgta
      181 ttaatttcga ttatgcggcc gtgtacaata cgattacttt ctgttcgact taagcattat
      241 gcggccgcaa gcttggatcc tcattcattc agtgatgatg atggtgatga gaagaacctc
      301 aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa ttctcgtcgc aaaatttttc
      361 cgacgttaga tttcggtaac tcatcacgaa actccaccag cttcggtact ttgtagcccg
      421 tgagctgacg gcggcaaaag gtcaccagtg actcttcggt aagcgatgga tcttttttca
      481 ctacgaagat tttcaccgct tcaccactgg agccggaagg tacgccaaca gccgcgactt
      541 cctgtacgcc aggatgctgc atgacgacat cttcaatctc gttgggatag acgttaaaac
      601 cggaaaccag aatcatgtct tttttacgat cgacaatgcg caggaaccct tcttcatcca
      661 tcaccgcgat gtcgccggtg tgtaaccagc catttttgat gatctcatct gtagcatccg
      721 gacgctgcca gtaacccagc atcacctgcg gtcctttgac acaaagctca cccggttgac
      781 ccggtggtac ttcattatca tcatcatcca ccagtttggc ttccgtcgac ggcaccggca
      841 aaccgatgct accactatga taatcaatac catatgggtt aacgctgacc agcggcgcac
      901 actcggtaag gccatagcct tccagcagat actgtcctgt cagtttcacc caacgctctg
      961 ccaccacttg ctgcactggc atccctccgc ctgcggaaag atgcagactg gagaaatcca
     1021 gctgctggaa ctcttt
//
