LOCUS       sequence-114690-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD H376R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS502 (TJF065)
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ggcccgatgc gtccggcgta gaggatcgag atcgatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacca tggcattgaa gaaggtttgg cttaaccgtt atcccgcgga
      181 cgttccgacg gagatcaacc ctgaccgtta tcaatctctg gtagatatgt ttgagcagtc
      241 ggtcgcgcgc tacgccgatc aacctgcgtt tgtgaatatg ggggaggtaa tgaccttccg
      301 caagctggaa gaacgcagtc gcgcgtttgc cgcttatttg caacaagggt tggggctgaa
      361 gaaaggcgat cgcgttgcgt tgatgatgcc taatttattg caatatccgg tggcgctgtt
      421 tggcattttg cgtgccggga tgatcgtcgt aaacgttaac ccgttgtata ccccgcgtga
      481 gcttgagcat cagcttaacg atagcggcgc atcggcgatt gttatcgtgt ctaactttgc
      541 tcacacactg gaaaaagtgg ttgataaaac cgccgttcag cacgtaattc tgacccgtat
      601 gggcgatcag ctatctacgg caaaaggcac ggtagtcaat ttcgttgtta aatacatcaa
      661 gcgtttggtg ccgaaatacc atctgccaga tgccatttca tttcgtagcg cactgcataa
      721 cggctaccgg atgcagtacg tcaaacccga actggtgccg gaagatttag cttttctgca
      781 atacaccggc ggcaccactg gtgtggcgaa aggcgcgatg ctgactcacc gcaatatgct
      841 ggcgaacctg gaacaggtta acgcgaccta tggtccgctg ttgcatccgg gcaaagagct
      901 ggtggtgacg gcgctgccgc tgtatcacat ttttgccctg accattaact gcctgctgtt
      961 tatcgaactg ggtgggcaga acctgcttat cactaa
//
