LOCUS       sequence-114700-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral expression of humanized ChR2 with E123T/H134R 
            mutations fused to EYFP driven by CaMKIIa promoter for optogenetic 
            activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Gunaydin LA, Yizhar O, Berndt A, Sohal VS, Deisseroth K, Hegemann P
  TITLE     Ultrafast optogenetic control.
  JOURNAL   Nat Neurosci. 2010 Mar;13(3):387-92. doi: 10.1038/nn.2495.
  PUBMED    20081849
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn"; 
            journalName: "Nat Neurosci"; date: "2010-03"; volume: "13"; issue: 
            "3"; pages: "387-92"
COMMENT     Sequence Label: pLenti-CaMKIIa-ChETA-EYFP
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(33..746,747..749,750..752))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(78..99)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     427..446
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     686..707
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      749..758
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggttgattat cgataagctt gatatcgaat tcttacttgt acagctcgtc catgccgaga
       61 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
      121 tctttgctca gggcggactg gtagctcagg tagtggttgt cgggcagcag cacggggccg
      181 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg
      241 ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag
      301 acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
      361 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
      421 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct
      481 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag
      541 cactgcaggc cgtagccgaa ggtggtcacg agggtgggcc agggcacggg cagcttgccg
      601 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg
      661 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
      721 ccggtgaaca gctcctcgcc cttgctcacc atggtggcgg ccgctggcac ggctccggcc
      781 tcggcttcgt cttcgacgag agtctcgacc tcgatctccg
//
