LOCUS       sequence-114703-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing the calcium indicator GCaMP6 driven by 
            the muscle-specific promoter MHCK7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Madden L, Juhas M, Kraus WE, Truskey GA, Bursac N
  TITLE     Bioengineered human myobundles mimic clinical responses of skeletal 
            muscle to drugs.
  JOURNAL   eLife. 2015 Jan 9;4:e04885. doi: 10.7554/eLife.04885.
  PUBMED    25575180
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2015-01-9"; volume: "4:e04885. doi"; pages: " 
            10.7554/eLife.04885"
COMMENT     Sequence Label: pRRL-MHCK7-GCaMP6
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     225..246
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(352..373)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(586..642)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      654..663
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(670..684,685..693))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(697..729)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(733..750)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      756..765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcgctcctgg atgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
       61 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
      121 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
      181 atcgccctca ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
      241 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgctccctcc
      301 ggtaccgccc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
      361 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttc gaaagtttgg actgcacgct
      421 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
      481 gtggtaggcg agctgcacgc cgccgtcctc gatgttgtgg cggatcttga agttcgcctt
      541 gatgccgttc ttctgcttgt cggccttgat atagacgttc tcgagtgagc tcagccgacc
      601 tatagctctg actgcgtgac ctgtcttatt ccacttacga cgtgatgagt cgaccatggt
      661 ggcgagatcc ttatcgtcat cgtcgtacag atcccgaccc atttgctgtc caccagtcat
      721 gctagccata ccatgatgat gatgatgatg agaacccatg gtggcnagat cgaccggtcg
      781 aggctggctg gctcctgagt
//
