LOCUS       sequence-114705-         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing the calcium indicator GCaMP6 driven by 
            the muscle-specific promoter MHCK7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Madden L, Juhas M, Kraus WE, Truskey GA, Bursac N
  TITLE     Bioengineered human myobundles mimic clinical responses of skeletal 
            muscle to drugs.
  JOURNAL   eLife. 2015 Jan 9;4:e04885. doi: 10.7554/eLife.04885.
  PUBMED    25575180
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2015-01-9"; volume: "4:e04885. doi"; pages: " 
            10.7554/eLife.04885"
COMMENT     Sequence Label: pRRL-MHCK7-GCaMP6
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..66)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      78..87
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(94..108,109..117))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(121..153)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(157..174)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      180..189
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gttctcgagt gagctcagcc gacctatagc tctgactgcg tgacctgtct tattccactt
       61 acgacgtgat gagtcgacca tggtggcgag atccttatcg tcatcgtcgt acagatcccg
      121 acccatttgc tgtccaccag tcatgctagc cataccatga tgatgatgat gatgagaacc
      181 catggtggcg agatcgaccg gtcgaggctg gctggctcct gagtgtctgt ctgtgctgtg
      241 gaggtggtgg tagaatgagg gcagcccctg tgcccctggg ttatatagag gagcctacag
      301 ggtgtgacta gccaggaggg gctgtcccca gggaggggcc cctgagagca gatgagcttt
      361 cagctcgttg cccgggcacc gtgcccaccc cggacccagg cgtgcagctt gcccagcccc
      421 atggccttgt atgggctgcc ccaagggctg acttgctcac tggttcctaa actaagtgct
      481 gagtctagct ggcgggggac agctggccct tcgccgggaa catggaacag taatactttg
      541 ggagtcccag gcacgtataa gccctggccc ccaagcctgt tacagcctgc cctcagtccc
      601 ccacagcctt gttcgaagat cttcgcatgc aggggatcca ccagggacag ggttattttt
      661 agaggcagca ggtgttgggg gggggggggc
//
