LOCUS       sequence-114710-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo visualization of the mitochondria (can be used for 
            colocalization studies).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen 
            L, Hink MA, van Weeren L, Gadella TW Jr, Royant A
  TITLE     Structure-guided evolution of cyan fluorescent proteins towards a 
            quantum yield of 93%.
  JOURNAL   Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/ncomms1738.
  PUBMED    22434194
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-03-20"; volume: "3:751. doi"; pages: " 
            10.1038/ncomms1738"
COMMENT     Sequence Label: pmTurquoise2-Mito
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             74..148
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      176..185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(182..184,185..187,188..898)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(227..248)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(488..507)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     835..856
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcatgtccg tcctgacgcc gctgctgctg cggggcttga caggctcggc
      121 ccggcggctc ccagtgccgc gcgccaagat ccattcgttg ggggatccac cggtcgccac
      181 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      241 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      301 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      361 cctcgtgacc accctgtcct ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa
      421 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      481 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      541 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      601 caagctggag tacaactact ttagcgacaa cgtctatatc accgccgaca agcagaagaa
      661 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc
      721 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      781 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
      841 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc
      901 cggactcaga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg
//
