LOCUS       sequence-114716-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo visualization of actin cytoskeleton (can be used for 
            colocalization studies).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen 
            L, Hink MA, van Weeren L, Gadella TW Jr, Royant A
  TITLE     Structure-guided evolution of cyan fluorescent proteins towards a 
            quantum yield of 93%.
  JOURNAL   Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/ncomms1738.
  PUBMED    22434194
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-03-20"; volume: "3:751. doi"; pages: " 
            10.1038/ncomms1738"
COMMENT     Sequence Label: pLifeAct-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    117..238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(128..147)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     182..201
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(364..615)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(406..427)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     755..774
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
       61 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt acgccttaag
      121 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
      181 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
      241 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
      301 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt cgcggccgct
      361 ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
      421 gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg tgctcaggta
      481 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
      541 ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc ttgaagttgg ccttgatgcc
      601 gttcttctgc ttgtcggcgg tgatatagac gttgtcgcta aagtagttgt actccagctt
      661 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
      721 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
      781 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
      841 cttcatgtgg tcggggtagc gggcgaagca ctgcacgccc caggacaggg tggtcacgag
      901 ggtgggccag ggcacg
//
