LOCUS       sequence-114726-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector (CMV promoter) of PercevalHR, a 
            fluorescent sensor of ATP-to-ADP ratio..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Tantama M, Martinez-Francois JR, Mongeon R, Yellen G
  TITLE     Imaging energy status in live cells with a fluorescent biosensor of 
            the intracellular ATP-to-ADP ratio.
  JOURNAL   Nat Commun. 2013;4:2550. doi: 10.1038/ncomms3550.
  PUBMED    24096541
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2550. doi"; pages: " 
            10.1038/ncomms3550"
COMMENT     Sequence Label: GW1-PercevalHR
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     204..225
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(286..537)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSFQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(328..349)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca
       61 ttggagaccg tagcccaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
      121 ggtgcagatc agcttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
      181 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc
      241 ggtgaacagc tcctcgccct tgctcaccat gcctccggaa ccgcccttgt acagctcgtc
      301 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
      361 ctcgttgggg tctttgctca gcttggactg gaagctcagg tagtggttgt cgggcagcag
      421 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgccgcc
      481 gtcctcgatg ttgtggcgga tcttgaagtt ggccttgatg ccgttcttct gcttgtcggc
      541 ggtgatgtag acgttgtcgc tgttgtagcc tgcagagtac acccttcctc ggtacctctc
      601 gaagatgccg ccctggacgc cagagccttt gacttcagag acggtcatac ccacatatcc
      661 agcgtccgag agagccttct taacgatctc cagcttttcg ggcctgatga tggattcaac
      721 ctttttcatg gtggtctaga ggcctcaggc acacctgctc cgccgatggt ggacgtggac
      781 agggaacgct ctcggacgcg gcactcactc cgattcagaa tctttatcgg tatcggactc
      841 ggatgcggat
//
