LOCUS       sequence-114727-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector (CMV promoter) of PercevalHR, a 
            fluorescent sensor of ATP-to-ADP ratio..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Tantama M, Martinez-Francois JR, Mongeon R, Yellen G
  TITLE     Imaging energy status in live cells with a fluorescent biosensor of 
            the intracellular ATP-to-ADP ratio.
  JOURNAL   Nat Commun. 2013;4:2550. doi: 10.1038/ncomms3550.
  PUBMED    24096541
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2550. doi"; pages: " 
            10.1038/ncomms3550"
COMMENT     Sequence Label: GW1-PercevalHR
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(85..106)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(346..365)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagggcggt tccggaggca tggtgagcaa gggcgaggag
       61 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      121 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg
      181 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac
      241 ggtctccaat gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      301 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      361 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      421 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agcttgagta caacggcacc
      481 atagtagatc tgatccctaa ggtaaaaatt gaactagtgg tgaaggagga ggacgtcgat
      541 aacgtgatag acattatttg cgaaaatgcc cgcacaggta accccggcga cggtaagata
      601 tttgtgatcc cagtggagcg agtggtcagg gtgcgaacca aagaggaggg agcatctggt
      661 ggtggatccg gtggtggcgg tgcatctggt atgaaaaagg ttgaagctat tattcgccca
      721 gagaaactgg agattgttaa gaaggccctg tccgacgccg gctatgttgg catgaccgtt
      781 tccgaggtta aaggccgcgg tgccggcggt ggcgacctga ttcctaaggt gaagatcgag
      841 ctggt
//
