LOCUS       sequence-114730-        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD V451A-His.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS533 (TJF076)
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    141..165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(165..184)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(166..184)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(273..290)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tggcgttcaa atttcgcagc agcggtttct ttaccagact cgagggtacc gacgtcagcg
       61 atcgcgtggc cggccgatat ccaattgaga tctgccatat gtatatctcc ttcttatact
      121 taactaatat actaagatgg ggaattgtta tccgctcaca attcccctat agtgagtcgt
      181 attaatttcg attatgcggc cgtgtacaat acgattactt tctgttcgac ttaagcatta
      241 tgcggccgca agcttggatc ctcattcatt cagtgatgat gatggtgatg agaagaaccg
      301 gctttattgt ccactttgcc gcgcgcttcg tcacgtaatt ctcgtcgcaa aatttttccg
      361 acgttagatt tcggtaactc atcacgaaac tccaccagct tcggtacttt gtagcccgtg
      421 agctgacggc ggcaaaaggt caccagtgac tcttcggtaa gcgatggatc ttttttcact
      481 acgaagattt tcaccgcttc accactggag ccggaaggta cgccaacagc cgcgacttcc
      541 tgtacgccag gatgctgcat gacgacatct tcaatctcgt tgggatagac gttaaaaccg
      601 gaaaccagaa tcatgtcttt tttacgatcg gcaatgcgca ggaacccttc ttcatccatc
      661 accgcgatgt cgccggtgtg taaccagcca tttttgatga tctcatctgt agcatccgga
      721 cgctgccagt aacccagcat cacctgcggt cctttgacac aaagctcacc cggttgaccc
      781 ggtggtactt cattatcatc atcatccacc agtttggctt ccgtcgacgg caccggcaaa
      841 ccgatgctac cactatgata atcaatatca tatgggttaa cgctgaccag cggcgcacac
      901 tcggtaaggc catagccttc cagcagatac tgtcctgtca gtttcaccca acgctctgcc
      961 accacttgct gcactggcat ccctccgcct gcggaaagat gcagactgga gaaatccagc
     1021 tgctggaact ctttattgtt cagcaacgca tt
//
