LOCUS       sequence-114737-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD-His.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS528 (TJF059)
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    146..170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(170..189)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(171..189)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(278..295)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgtgctggcg ttcaaatttc gcagcagcgg tttctttacc agactcgagg gtaccgacgt
       61 cagcgatcgc gtggccggcc gatatccaat tgagatctgc catatgtata tctccttctt
      121 atacttaact aatatactaa gatggggaat tgttatccgc tcacaattcc cctatagtga
      181 gtcgtattaa tttcgattat gcggccgtgt acaatacgat tactttctgt tcgacttaag
      241 cattatgcgg ccgcaagctt ggatcctcat tcattcagtg atgatgatgg tgatgagaag
      301 aaccggcttt attgtccact ttgccgcgcg cttcgtcacg taattctcgt cgcaaaattt
      361 ttccgacgtt agatttcggt aactcatcac gaaactccac cagcttcggt actttgtagc
      421 ccgtgagctg acggcggcaa aaggtcacca gtgactcttc ggtaagcgat ggatcttttt
      481 tcactacgaa gattttcacc gcttcaccac tggagccgga aggtacgcca acagccgcga
      541 cttcctgtac gccaggatgc tgcatgacga catcttcaat ctcgttggga tagacgttaa
      601 aaccggaaac cagaatcatg tcttttttac gatcgacaat gcgcaggaac ccttcttcat
      661 ccatcaccgc gatgtcgccg gtgtgtaacc agccattttt gatgatctca tctgtagcat
      721 ccggacgctg ccagtaaccc agcatcacct gcggtccttt gacacaaagc tcacccggtt
      781 gacccggtgg tacttcatta tcatcatcat ccaccagttt ggcttccgtc gacggcaccg
      841 gcaaaccgat gctaccacta tgataatcaa tatcatatgg gttaacgctg accagcggcg
      901 cacactcggt aaggccatag ccttccagca gatactgtcc tgtcagtttc acccaacgct
      961 ctgccaccac ttgctgcact ggcatccctc cgcctgcgga aagatgcaaa ctggagaaat
     1021 ccagctgctg gaact
//
