LOCUS       sequence-114745-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-HA expression vector with RBPMS carrying mutation F27A and 
            encoding silent mutation to render resistant to siRNA R2 (Farazi et 
            al. 2014).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_KDR_F27A
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     38..62
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             162..185
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             186..212
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    228..252
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga
       61 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      121 gcctccggac tctagcgttt aaacttaagc ttggtaccat ggactacaag gacgacgatg
      181 acaagtaccc ttatgacgtg cccgattacg ctttgaacca gatatcaaca agtttgtaca
      241 aaaaagcagg ctccaccatg ggaaccaatt cagtcgacat gaacaacggc ggcaaagccg
      301 agaaggagaa caccccgagc gaggccaacc ttcaggagga ggaggtccgg accctagctg
      361 tcagtggcct tcctctggac attaaacctc gggagctcta tctgcttttc agaccattta
      421 agggctatga gggttctctt ataaagctca catctaaaca gcctgtaggt tttgtcagtt
      481 ttgacagtcg ctcagaagca gaggctgcaa agaatgcttt gaatggcatc cgcttcgatc
      541 ctgaaattcc gcaaacacta cgactagagt ttgctaaggc aaacacgaag atggccaaga
      601 acaaactcgt agggactcca aaccccagta ctcctctgcc caacactgta cctcagttca
      661 ttgccagaga gccatatgag ctcacagtgc ctgcacttta ccccagtagc cctgaagtgt
      721 gggccccgta ccctctgtac ccagcggagt tagcgcctgc tctacctcct cctgctttca
      781 cctatcccgc ttcactgcat gcccagatgc gctggctccc
//
