LOCUS       sequence-114746-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-HA expression vector with RBPMS carrying mutation F27A and 
            encoding silent mutation to render resistant to siRNA R2 (Farazi et 
            al. 2014).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_KDR_F27A
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..72)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    125..149
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(790..814)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agttatctat gcggccgcgg atcaggtacg cgtagaatcg agaccgagga gagggttagg
       61 gataggctta ccttcgaacc gcgggccctc tagacttgag cggccgccac tgtgctggat
      121 atcaaccact ttgtacaaga aagctgggtc tagatatctc gagtgcggcc gctcagcaga
      181 actgacggga cttccagccc tgagaagtag cctcggaggg agggagccag cgcatctggg
      241 catgcagtga agcgggatag gtgaaagcag gaggaggtag agcaggcgct aactccgctg
      301 ggtacagagg gtacggggcc cacacttcag ggctactggg gtaaagtgca ggcactgtga
      361 gctcatatgg ctctctggca atgaactgag gtacagtgtt gggcagagga gtactggggt
      421 ttggagtccc tacgagtttg ttcttggcca tcttcgtgtt tgccttagca aactctagtc
      481 gtagtgtttg cggaatttca ggatcgaagc ggatgccatt caaagcattc tttgcagcct
      541 ctgcttctga gcgactgtca aaactgacaa aacctacagg ctgtttagat gtgagcttta
      601 taagagaacc ctcatagccc ttaaatggtc tgaaaagcag atagagctcc cgaggtttaa
      661 tgtccagagg aaggccactg acagctaggg tccggacctc ctcctcctga aggttggcct
      721 cgctcggggt gttctccttc tcggctttgc cgccgttgtt catgtcgact gaattggttc
      781 ccatggtgga gcctgctttt ttgtacaaac ttgttgatat
//
