LOCUS       sequence-114750-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-HA expression vector with RBPMS carrying mutation E97A, K100A 
            and encoding silent mutation to render resistant to siRNA R2 (Farazi
            et al. 2014).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_KDR_E97A_K100A
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(37..78)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    131..155
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(796..820)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tggatcagtt atctatgcgg ccgcggatca ggtacgcgta gaatcgagac cgaggagagg
       61 gttagggata ggcttacctt cgaaccgcgg gccctctaga cttgagcggc cgccactgtg
      121 ctggatatca accactttgt acaagaaagc tgggtctaga tatctcgagt gcggccgctc
      181 agcagaactg acgggacttc cagccctgag aagtagcctc ggagggaggg agccagcgca
      241 tctgggcatg cagtgaagcg ggataggtga aagcaggagg aggtagagca ggcgctaact
      301 ccgctgggta cagagggtac ggggcccaca cttcagggct actggggtaa agtgcaggca
      361 ctgtgagctc atatggctct ctggcaatga actgaggtac agtgttgggc agaggagtac
      421 tggggtttgg agtccctacg agtttgttct tggccatctt cgtgtttgcc gcagcaaacg
      481 ctagtcgtag tgtttgcgga atttcaggat cgaagcggat gccattcaaa gcattctttg
      541 cagcctctgc ttctgagcga ctgtcaaaac tgacaaaacc tacaggctgt ttagatgtga
      601 gctttataag agaaccctca tagcccttaa atggtctgaa aagcagatag agctcccgag
      661 gtttaatgtc cagaggaagg ccactgacaa atagggtccg gacctcctcc tcctgaaggt
      721 tggcctcgct cggggtgttc tccttctcgg ctttgccgcc gttgttcatg tcgactgaat
      781 tggttcccat ggtggagcct gcttttttgt acaaacttgt t
//
