LOCUS       sequence-114752-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Hartmann C, Tabin CJ
  TITLE     Dual roles of Wnt signaling during chondrogenesis in the chicken 
            limb.
  JOURNAL   Development. 2000 Jul . 127(14):3141-59.
  PUBMED    10862751
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2000 Jul . 127(14):3141-59."
COMMENT     Sequence Label: pGEM cOp (CT#625)
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             528..545
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(822..840)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(823..840)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(858..874)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(858..874)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    883..899
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aagggcgaat tgggcccgac gtcgcatgct cccggccgcc atggcggccg cgggaattcg
       61 attgttttca atgctgtggc gatcttcggc atcctcagcg ctctctagcg tctggttgct
      121 ggtgtcgacg gcggacgcgc tagccaggct ctctctgctg tccaccccag cactggcctt
      181 gctgtctcct ccgtccacct cagggctgtc aaatcttggc cggctgtcgt tctctacgct
      241 ctgatgttgg gccagctcac ggctgtcctg ttcgcggctc tccttgggca accagaggcc
      301 cgctagctgg ctgtccccaa cctccgcggt ggcgtcatcc tcaatgagct tcctggcagc
      361 tttgcggagc ttgctcgcct tcaccacgtg ggctttggcc ctgaagccat atgccacact
      421 gtcaccccgg cctgcgttgt ccccacggtt gaaaggtgtc acgggtgcct ctgtggggaa
      481 gtctgtgacg acttcatcag actcgtcagt gtcattggaa tcattgtcat catcatcatc
      541 atcatccatg gcctcttggc tcttgctagg aatgtcagga aagtccggct gttctgggac
      601 atcagcattt tcttctgaag agtaatgagt ctgctgaagt gaagccaggt cattctgtgt
      661 ctgctgcagg tgctcctgag actgagaatc cacgtgatcc tggtggtacc tgtgtgtgtg
      721 gtggctcctg gggtcgtatt taatcactag tgaattcgcg gccgcctgca ggtcgaccat
      781 atgggagagc tcccaacgcg ttggatgcat agcttgagta ttctatagtg tcacctaaat
      841 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa aattgttatc cgctcacaaa
      901 ttccacacaa catacgaagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
//
