LOCUS       sequence-114755-         822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FLAG-HA expression vector with RBPMS carrying mutation K36A, R38A, 
            E39A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_K36A_R38A_E39A
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    12..30
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     40..64
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             164..187
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             188..214
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    230..254
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca
       61 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      121 cagcctccgg actctagcgt ttaaacttaa gcttggtacc atggactaca aggacgacga
      181 tgacaagtac ccttatgacg tgcccgatta cgctttgaac cagatatcaa caagtttgta
      241 caaaaaagca ggctccacca tgggaaccaa ttcagtcgac atgaacaacg gcggcaaagc
      301 cgagaaggag aacaccccga gcgaggccaa ccttcaggag gaggaggtcc ggaccctatt
      361 tgtcagtggc cttcctctgg acattgcacc tgcggcgctc tatctgcttt tcagaccatt
      421 taagggctat gagggttctc ttataaagct cacatctaaa cagcctgtag gttttgtcag
      481 ttttgacagt cgctcagaag cagaggctgc aaagaatgct ttgaatggca tccgcttcga
      541 tcctgaaatt ccgcaaacac tacgactaga gtttgctaag gcaaacacga agatggccaa
      601 gaacaaactc gtagggactc caaaccccag tactcctctg cccaacactg tacctcagtt
      661 cattgccaga gagccatatg agctcacagt gcctgcactt taccccagta gccctgaagt
      721 gtgggccccg taccctctgt acccagcgga gttagcgcct gctctacctc ctcctgcttt
      781 cacctatccc gcttcactgc atgcccagat gcgctggctc cc
//
