LOCUS       sequence-114760-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-HA expression vector with RBPMS carrying mutation R38A, E39A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_R38A_E39A
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..80)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    133..157
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(798..822)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actggatcag ttatctatgc ggccgcggat caggtacgcg tagaatcgag accgaggaga
       61 gggttaggga taggcttacc ttcgaaccgc gggccctcta gacttgagcg gccgccactg
      121 tgctggatat caaccacttt gtacaagaaa gctgggtcta gatatctcga gtgcggccgc
      181 tcagcagaac tgacgggact tccagccctg agaagtagcc tcggagggag ggagccagcg
      241 catctgggca tgcagtgaag cgggataggt gaaagcagga ggaggtagag caggcgctaa
      301 ctccgctggg tacagagggt acggggccca cacttcaggg ctactggggt aaagtgcagg
      361 cactgtgagc tcatatggct ctctggcaat gaactgaggt acagtgttgg gcagaggagt
      421 actggggttt ggagtcccta cgagtttgtt cttggccatc ttcgtgtttg ccttagcaaa
      481 ctctagtcgt agtgtttgcg gaatttcagg atcgaagcgg atgccattca aagcattctt
      541 tgcagcctct gcttctgagc gactgtcaaa actgacaaaa cctacaggct gtttagatgt
      601 gagctttata agagaaccct catagccctt aaatggtctg aaaagcagat agagcgccgc
      661 aggtttaatg tccagaggaa ggccactgac aaatagggtc cggacctcct cctcctgaag
      721 gttggcctcg ctcggggtgt tctccttctc ggctttgccg ccgttgttca tgtcgactga
      781 attggttccc atggtggagc ctgctttttt gtacaaactt gttgatatct
//
