LOCUS       sequence-114766-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD T349A-His.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS536 (TJF079)
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..49)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    158..182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(182..201)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(183..201)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(290..307)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagacgagtc catgtgctgg cgttcaaatt tcgcagcagc ggtttcttta ccagactcga
       61 gggtaccgac gtcagcgatc gcgtggccgg ccgatatcca attgagatct gccatatgta
      121 tatctccttc ttatacttaa ctaatatact aagatgggga attgttatcc gctcacaatt
      181 cccctatagt gagtcgtatt aatttcgatt atgcggccgt gtacaatacg attactttct
      241 gttcgactta agcattatgc ggccgcaagc ttggatcctc attcattcag tgatgatgat
      301 ggtgatgaga agaaccggct ttattgtcca ctttgccgcg cgcttcgtca cgtaattctc
      361 gtcgcaaaat ttttccgacg ttagatttcg gtaactcatc acgaaactcc accagcttcg
      421 gtactttgta gcccgtgagc tgacggcggc aaaaggtcac cagtgactct tcggtaagcg
      481 atggatcttt tttcactacg aagattttca ccgcttcacc actggagccg gaaggtacgc
      541 caacagccgc gacttcctgt acgccaggat gctgcatgac gacatcttca atctcgttgg
      601 gatagacgtt aaaaccggaa accagaatca tgtctttttt acgatcgaca atgcgcagga
      661 acccttcttc atccatcacc gcgatgtcgc cggtgtgtaa ccagccattt ttgatgatct
      721 catctgtagc atccggacgc tgccagtaac ccagcatcac ctgcggtcct ttgacacaaa
      781 gctcacccgg ttgacccggt ggtacttcat tatcatcatc atccaccagt ttggcttccg
      841 tcgacggcac cggcaaaccg atgctaccac tatgataatc aatatcatat gggttaacgc
      901 tgaccagcgg cgcacactcg gtaaggccat agccttccag cagatactgt cctgccagtt
      961 tcacccaacg ctctgccacc acttgctgca ctggcatccc tccgcctgcg gaaagatgca
     1021 aactggagaa atccagctgc tggaactctt tattgttcag ca
//
