LOCUS       sequence-114779-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD Y375R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS511 (TJF032 Y375R)
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    76..100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             115..137
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccggccacga atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg cgaaattaat
       61 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
      121 taactttaag aaggagatat accatggcat tgaagaaggt ttggcttaac cgttatcccg
      181 cggacgttcc gacggagatc aaccctgacc gttatcaatc tctggtagat atgtttgagc
      241 agtcggtcgc gcgctacgcc gatcaacctg cgtttgtgaa tatgggggag gtaatgacct
      301 tccgcaagct ggaagaacgc agtcgcgcgt ttgccgctta tttgcaacaa gggttggggc
      361 tgaagaaagg cgatcgcgtt gcgttgatga tgcctaattt attgcaatat ccggtggcgc
      421 tgtttggcat tttgcgtgcc gggatgatcg tcgtaaacgt taacccgttg tataccccgc
      481 gtgagcttga gcatcagctt aacgatagcg gcgcatcggc gattgttatc gtgtctaact
      541 ttgctcacac actggaaaaa gtggttgata aaaccgccgt tcagcacgta attctgaccc
      601 gtatgggcga tcagctatct acggcaaaag gcacggtagt caatttcgtt gttaaataca
      661 tcaagcgttt ggtgccgaaa taccatctgc cagatgccat ttcatttcgt agcgcactgc
      721 ataacggcta ccggatgcag tacgtcaaac ccgaactggt gccggaagat ttagcttttc
      781 tgcaatacac cggcggcacc actggtgtgg cgaaaggcgc gatgctgact caccgcaata
      841 tgctggcgaa cctggaacag gttaacgcga cctatggtcc gctgttgcat ccgggcaaag
      901 agctggtggt gacggcgctg ccgctgtatc acatttttgc cctgaccatt aactgcctgc
      961 tgtttatcga actgggtggg cagaacctgc ttatcactaa cccgcgcgat attccagggt
     1021 tggtaaaaga attagcgaaa tatccgttta ccgc
//
