LOCUS       sequence-11478-e        6658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Carroll P, Schreuder LJ, Muwanguzi-Karugaba J, Wiles S, Robertson 
            BD, Ripoll J, Ward TH, Bancroft GJ, Schaible UE, Parish T
  TITLE     Sensitive detection of gene expression in mycobacteria under 
            replicating and non-replicating conditions using optimized far-red 
            reporters.
  JOURNAL   PLoS One. 2010  . 5(3):e9823.
  PUBMED    20352111
REFERENCE   2  (bases 1 to 6658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(3):e9823."
COMMENT     Sequence Label: pCHERRY8
FEATURES             Location/Qualifiers
     source          1..6658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1387..1403)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1388..1404)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     rep_origin      5623..6211
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6112..6131
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6365..6382
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6499..6520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6535..6540,6541..6558,6559..6565)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6573..6589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6578..6600
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6597..6613
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6597..6613
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tctagagggt gcgatctacc gtcgctggcc gtcgaagatc gagatgaccg cggccgcgat
       61 gcgcaccctg gcgctgcccg agggcgcggc gccggacacc ggatcgttgt acggtgacgt
      121 cctggcgctc ctggcggacg tcaaccggtg gatcggcgac gaacgcatgc gccggctgta
      181 cccggatctg ctggccgagg cccagcgcaa cccggtgctg gcctaggcac tgatggacac
      241 cgtcggtacg ccgcgccggg cgcgtgcgcg cgatattctc gatcgcgccg ccactcgcgg
      301 agagcttgcc cccggtgccg actccgaact gctggtcgac gcgatgggcg cgctggtctt
      361 ctggcgtctg atcgcactgt ttcggcctgt gacgtcggaa tatctggatc gcgtcgcacg
      421 tgcgatctgt gcgttcgcac gcacaggccc ggtgtgagaa gggtctctga cgagcgggag
      481 aacccacccg gggtgggcga gtttgtcctg cgtgtgctcg gtcgagtagg ctctgggagt
      541 acccgtgtgt acgaccagca cggcatacat catttcgacg ccgagagatt cgccgcccga
      601 aatgagcacg atcaacaaaa gaactgtccc tacgattaca cctgtccgag caacccacca
      661 cggatccatg gtctcgaagg gcgaggagga caacatggcg atcatcaagg agttcatgcg
      721 cttcaaggtc cacatggagg gctcggtcaa cggccacgag ttcgagatcg agggcgaggg
      781 cgagggccgc ccgtacgagg gcacccagac cgccaagctg aaggtcacca agggcggccc
      841 gctgccgttc gcctgggaca tcctgtcgcc gcagttcatg tacggcagca aggcctacgt
      901 caagcacccg gccgacatcc cggactacct gaagctgtcg ttcccggagg gcttcaagtg
      961 ggagcgcgtc atgaacttcg aggacggcgg cgtcgtcacc gtcacccagg actcgtcgct
     1021 gcaggacggc gagttcatct acaaggtcaa gctgcggggc accaacttcc cgtcggacgg
     1081 cccggtcatg cagaagaaga ccatgggctg ggaggcctcg tcggagcgca tgtacccgga
     1141 ggacggcgcc ctgaagggcg agatcaagca gcggctgaag ctgaaggacg gcggccacta
     1201 cgacgccgag gtcaagacca cctacaaggc caagaagccg gtccagctgc cgggcgccta
     1261 caacgtgaac atcaagctgg acatcaccag ccacaacgag gactacacca tcgtcgagca
     1321 gtacgagcgc gccgagggcc gccacagcac cggcggcatg gacgagctgt acaagtgaaa
     1381 gcttatcgat accgtcgacc tcgagggggg gcccggtacg tacccgggga tcatcgagcc
     1441 gagaacgtta tcgaagttgg tcatgtgtaa tcccctcgtt tgaactttgg attaagcgta
     1501 gatacaccct tggacaagcc agttggattc ggagacaagc aaattcagcc ttaaaaaggg
     1561 cgaggcctgc ggtggtggaa caccgcaggg cctctaaccg ctcgacgcgc tgcaccaacc
     1621 agcccgcgaa cggctggcag ccagcgtaag gcgcggctca tcgggcggcg ttcgccacga
     1681 tgtcctgcac ttcgagccaa gcctcgaaca cctgctggtg tgcacgactc acccggttgt
     1741 tgacaccgcg cgcggccgtg cgggctcggt ggggcggctg tgtcgccctt gccagcgtga
     1801 gtagcgcgta cctcacctcg cccaacaggt cgcacacagc cgattcgtac gccataaagc
     1861 caggtgagcc caccagctcc gtaagttcgg gcgctgtgtg gctcgtaccc gcgcattcag
     1921 gcggcagggg gtctaacggg tctaaggcgg cgtgtacggc cgccacagcg gctctcagcg
     1981 gcccggaaac gtcctcgaaa cgacgcatgt gttcctcctg gttggtacag gtggttgggg
     2041 gtgctcggct gtcgctggtg ttccaccacc agggctcgac gggagagcgg gggagtgtgc
     2101 agttgtgggg tggcccctca gcgaaatatc tgacttggag ctcgtgtcgg accatacacc
     2161 ggtgattaat cgtggtctac taccaagcgt gagccacgtc gccgacgaat ttgagcagct
     2221 ctggctgccg tactggccgc tggcaagcga cgatctgctc gaggggatct accgccaaag
     2281 ccgcgcgtcg gccctaggcc gccggtacat cgaggcgaac ccaacagcgc tggcaaacct
     2341 gctggtcgtg gacgtagacc atccagacgc agcgctccga gcgctcagcg cccgggggtc
     2401 ccatccgctg cccaacgcga tcgtgggcaa tcgcgccaac ggccacgcac acgcagtgtg
     2461 ggcactcaac gcccctgttc cacgcaccga atacgcgcgg cgtaagccgc tcgcatacat
     2521 ggcggcgtgc gccgaaggcc ttcggcgcgc cgtcgacggc gaccgcagtt actcaggcct
     2581 catgaccaaa aaccccggcc acatcgcctg ggaaacggaa tggctccact cagatctcta
     2641 cacactcagc cacatcgagg ccgagctcgg cgcgaacatg ccaccgccgc gctggcgtca
     2701 gcagaccacg tacaaagcgg ctccgacgcc gctagggcgg aattgcgcac tgttcgattc
     2761 cgtcaggttg tgggcctatc gtcccgccct catgcggatc tacctgccga cccggaacgt
     2821 ggacggactc ggccgcgcga tctatgccga gtgccacgcg cgaaacgccg aattcccgtg
     2881 caacgacgtg tgtcccggac cgctaccgga cagcgaggtc cgcgccatcg ccaacagcat
     2941 ttggcgttgg atcacaacca agtcgcgcat ttgggcggac gggatcgtgg tctacgaggc
     3001 cacactcagt gcgcgccagt cggccatctc gcggaaggga gcagcgcgca cggcggcgag
     3061 cacagttgcg cggcgcgcaa agtccgcgtc agccatggag gcattgctat gagcgacggc
     3121 tacagcgacg gctacagcga cggctacaac cggcagccga ctgtccgcaa aaagcggcgc
     3181 gtgaccgccg ccgaaggcgc tcgaatcacc ggactatccg aacgccacgt cgtccggctc
     3241 gtggcgcagg aacgcagcga gtggctcgcc gagcaggctg cacgccgcga acgcatccgc
     3301 gcctatcacg acgacgaggg ccactcttgg ccgcaaacgg ccaaacattt cgggctgcat
     3361 ctggacaccg ttaagcgact cggctatcgg gcgaggaaag agcgtgcggc agaacaggaa
     3421 gcggctcaaa aggcccacaa cgaagccgac aatccaccgc tgttctaacg caattgggga
     3481 gcgggtgtcg cgggggttcc gtggggggtt ccgttgcaac gggtcggaca ggtaaaagtc
     3541 ctggtagacg ctagttttct ggtttgggcc atgcctgtct cgttgcgtgt ttcgttgcgc
     3601 ccgttttgaa taccagccag acgagacggg gttctacgaa tcttggtcga taccaagcca
     3661 tttccgctga atatcgggga gctcaccgcc agaatcggtg gttgtggtga tgtacgtggc
     3721 gaactccgtt gtagtgcctg tggtggcatc cgtggccact ctcgttgcac ggttcgttgt
     3781 gccgttacag gccccgttga cagctcaccg aacgtagtta aaacatgctg gtcaaactag
     3841 gtttaccaac gatacgagtc agctcatcta gggccagttc taggcgttgt tcgttgcgcg
     3901 gttcgttgcg catgtttcgt gtggttgcta gatggctccg caaccacacg cttcgaggtt
     3961 gagtgcttcc agcacgggcg cgatccagaa gaacttcgtc gtgcgactgt cctcgttgat
     4021 ccttgccgag ctgggatgga agctcggccg accaccctgg aggagatgat cgaggatgcc
     4081 agggcctttc acgcccgccg ctgctgagcg tccgccgccg ggcccgcacc gccgtcggcc
     4141 ggcccgctcc gggctcgcag cagcgggctt cggcgcgggc ccggggctcc cgagcgcggg
     4201 cggggctccg ggcggccgcc gggggccggg ggcggcgccg ggcggcccgg ggcgtcaggc
     4261 gccgggggcg gtgtccggcg gcccccagag gaactgcgcc agttcctccg gatcggtgaa
     4321 gccggagaga tccagcgggg tctcctcgaa cacctcgaag tcgtgcagga aggtgaaggc
     4381 gagcagttcg cgggcgaagt cctcggtccg cttccactgc gccccgtcga gcagcgcggc
     4441 caggatctcg cggtcgcccc ggaaggcgtt gagatgcagt tgcaccaggc tgtagcggga
     4501 gtctcccgca tagacgtcgg tgaagtcgac gatcccggtg acctcggtcg cggccaggtc
     4561 cacgaagatg ttggtcccgt gcaggtcgcc gtggacgaac cggggttcgc ggccggccag
     4621 cagcgtgtcc acgtccggca gccagtcctc caggcggtcc agcagccggg gcgagaggta
     4681 gccccacccg cggtggtcct cgacggtcgc cgcgcggcgt tcccgcagca gttccgggaa
     4741 gacctcggaa tggggggtga gcacggtgtt cccggtcagc ggcaccctgt gcagccggcc
     4801 gagcacccgg ccgagttcgc gggccagggc gagcagcgcg ttccggtcgg tcgtgccgtc
     4861 catcgcggac cgccaggtgg tgccggtcat ccggctcatc accaggtagg gccacggcca
     4921 ggctccggtg ccgggccgca gctcgccgcg gccgaggagg cggggcaccg gcaccggggc
     4981 gtccgccagg accgcgtacg cctccgactc cgacgcgagg ctctccggac cgcaccagtg
     5041 ctcgccgaac agcttgatca ccgggtcggg ctcgccgacc agtacggggt tggtgctctc
     5101 gccgggcacc cgcagcaccg gcggcaccgg cagcccgagc tcctccaggg ctcggcgggc
     5161 cagcggctcc cagaattcct ggtcgttccg caggctcgcg taggaatcat ccgaatcaat
     5221 acggtcgaga agtaacaggg attcttgtgt cacagcggac ctctattcac agggtacggg
     5281 ccggcttaat tccgcacggc cggtcgcgac acggcctgtc cgcaccgcgg atcaggcgtt
     5341 gacgatgacg ggctggtcgg ccacgtcggg gacgacgggg agtcaggcaa ctatggatga
     5401 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5461 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5521 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5581 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5641 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5701 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5761 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5821 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5881 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5941 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6001 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6061 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6121 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6181 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6241 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6301 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     6361 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     6421 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     6481 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     6541 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     6601 aaacagctat gaccatgatt accagatctg gctcgagctc caccgcggtg gcggccgc
//
