LOCUS       sequence-114783-        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for expressing N-terminally Flag-tagged 
            short isoform of SadB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Eklund G, Lang S, Glindre J, Ehlen A, Alvarado-Kristensson M
  TITLE     The nuclear localization of gamma-tubulin is regulated by 
            SadB-mediated phosphorylation.
  JOURNAL   J Biol Chem. 2014 Aug 1;289(31):21360-73. doi: 
            10.1074/jbc.M114.562389. Epub 2014 Jun 18.
  PUBMED    24942739
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.562389"; journalName: "J Biol Chem"; date: 
            "2014-08-1- 1"; volume: "289"; issue: "31"; pages: "21360-73"
COMMENT     Sequence Label: pCDNA3 humanN-Flag SadBS
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..39
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcgaagctta ccatggatta taaagatgat gatgataaaa tgtcgtccgg ggccaaggag
       61 ggaggtgggg gctctcccgc ctaccacctc ccccaccccc acccccaccc accccagcac
      121 gcccaatatg tgggccccta tcggctggag aagacgctgg gcaaaggaca gacagggctg
      181 gttaaactcg gggtccactg catcacgggt cagaaggtcg ccatcaagat cgtgaaccgg
      241 gagaagctgt cggagtcggt gctgatgaag gtggagcggg agatcgccat cctgaagctc
      301 atcgaacacc cacatgtcct caagctccac gacgtctacg agaacaagaa atatttgtac
      361 ctggttctgg agcacgtctc ggggggtgag ctattcgact acctggtaaa gaaggggaga
      421 ctgacgccca aggaggcccg aaagttcttc cgccagattg tgtctgcgct ggacttctgc
      481 cacagctact ccatctgcca cagagaccta aagcccgaga acctgctttt ggatgagaaa
      541 aacaacatcc gcattgcaga cttcggcatg gcgtccctgc aggtggggga cagcctcctg
      601 gagaccagct gcgggtcccc ccattatgcg tgtccagagg tgattaaggg ggaaaaatat
      661 gatggccgcc gggcagacat gtggagctgt ggagtcatcc tcttcgccct gctcgtgggg
      721 gctctgccct ttgatgacga caacctccgc cagctgctgg agaaggtgaa acggggcgtc
      781 ttccacatgc cccacttcat tcctccagat tgccagagcc tcctgagggg aatgatcgaa
      841 gtggagcccg aaaaaaggct cagtctggag caaattcaga aacatccttg gtacctaggc
      901 gggaaacacg agccagaccc gtgcctggag ccagcccctg gccgccgggt agccatgcgg
      961 agcctgccat ccaacggaga gctggacccc gacgtcctag agagcatggc atcactgggc
     1021 tgcttcaggg accgcgagag gctgcatcgc gagctgcgca gtgaggagta agaattc
//
