LOCUS       sequence-114784-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Cx43 and EGFP seperately driven by the ECMV 
            IRES. For transfection or retroviral production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Salat-Canela C, Sese M, Peula C, Ramon y Cajal S, Aasen T
  TITLE     Internal translation of the connexin 43 transcript.
  JOURNAL   Cell Commun Signal. 2014 May 8;12:31. doi: 10.1186/1478-811X-12-31.
  PUBMED    24884945
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Commun
            Signal."; date: "2014-05-8"; volume: "12:31. doi"; pages: " 
            10.1186/1478-811X-12-31"
COMMENT     Sequence Label: pLPCX-Cx43-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..78
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     63..79
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgaggtcg acggtatcga taagcttgat atcaacaagt ttgtacaaaa aagcaggctt
      121 catgggtgac tggagcgcct taggcaaact ccttgacaag gttcaagcct actcaactgc
      181 tggagggaag gtgtggctgt cagtactttt cattttccga atcctgctgc tggggacagc
      241 ggttgagtca gcctggggag atgagcagtc tgcctttcgt tgtaacactc agcaacctgg
      301 ttgtgaaaat gtctgctatg acaagtcttt cccaatctct catgtgcgct tctgggtcct
      361 gcagatcata tttgtgtctg tacccacact cttgtacctg gctcatgtgt tctatgtgat
      421 gcgaaaggaa gagaaactga acaagaaaga ggaagaactc aaggttgccc aaactgatgg
      481 tgtcaatgtg gacatgcact tgaagcagat tgagataaag aagttcaagt acggtattga
      541 agagcatggt aaggtgaaaa tgcgaggggg gttgctgcga acctacatca tcagtatcct
      601 cttcaagtct atctttgagg tggccttctt gctgatccag tggtacatct atggattcag
      661 cttgagtgct gtttacactt gcaaaagaga tccctgccca catcaggtgg actgtttcct
      721 ctctcgcccc acggagaaaa ccatcttcat catcttcatg ctggtggtgt ccttggtgtc
      781 cctggccttg aatatcattg aactcttcta tgttttcttc aagggcgtta aggatcgggt
      841 taagggaaag agcgaccctt accatgcgac cagtggtgcg ctgagccctg ccaaagactg
      901 tgggtctcaa aaatatgctt atttcaatgg ctgctcctca ccaaccgctc ccctctcgcc
      961 tatgt
//
