LOCUS       sequence-114789-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Full length RCAN1 expression with CMV promoter.  Flag tagged..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Liu Q, Busby JC, Molkentin JD
  TITLE     Interaction between TAK1-TAB1-TAB2 and RCAN1-calcineurin defines a 
            signalling nodal control point.
  JOURNAL   Nat Cell Biol. 2009 Feb;11(2):154-61. doi: 10.1038/ncb1823. Epub 
            2009 Jan 11.
  PUBMED    19136967
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb1823"; 
            journalName: "Nat Cell Biol"; date: "2009-02"; volume: "11"; issue: 
            "2"; pages: "154-61"
COMMENT     Sequence Label: RCAN1
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    81..215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(105..124)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     159..178
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     regulatory      219..228
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(851..874)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tttccgcctc agaaggtacc taaccaagtt cctctttcag aggttatttc aggccatggt
       61 gctgcgccga attaattccc gatccagaca tgataagata cattgatgag tttggacaaa
      121 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
      181 tatttgtaac cattataagc tgcaataaac aagttgggcc atggcggcca agctttcagc
      241 taaggtggat cggtgtgtac tccggtctcc gtgtctggat gattttgggc ttgggtctct
      301 tcattctctc catctcttcc tcttcctcct cattctcttg gtcactctca cacacgtgga
      361 ccaccacact gggagtggtg tctgtcgctg catgcagttc atacttctct cctggcccca
      421 gcttggagat ggcatataaa agatcgtaat ttatgacggg ggtggcatct tctacttgtt
      481 tccagccaac gggaggagag gccggagggg agatgaggaa ctgtttgtcg ggattgggcg
      541 gagccaggtg tgaacttcct atgtgtaaag tctgagcaaa atacaacttc atttccttcc
      601 ccaggaactc ggtcttgtgc agccgcagcc tggcatcggc tgcagataag gggttgctga
      661 agtttatccg gacacgtttg aagctcttaa aatactggaa ggtggtgtcc ttgtcatatg
      721 ttctgaagag ggattcaaat ttggcccggc acaggccgtc cacgaacacg cgcgggtcca
      781 ggtggcaggc gatggtggcg ctcggcaggt cctgcagatc cacctcctcc atggatcccc
      841 ggggaattcg cttgtcgtca tcgtctttgt agtccatatc gatagaaccg aggtgcagtt
      901 ggacctggga
//
