LOCUS       sequence-11479-e        6459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Carroll P, Schreuder LJ, Muwanguzi-Karugaba J, Wiles S, Robertson 
            BD, Ripoll J, Ward TH, Bancroft GJ, Schaible UE, Parish T
  TITLE     Sensitive detection of gene expression in mycobacteria under 
            replicating and non-replicating conditions using optimized far-red 
            reporters.
  JOURNAL   PLoS One. 2010  . 5(3):e9823.
  PUBMED    20352111
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(3):e9823."
COMMENT     Sequence Label: pCHERRY10
FEATURES             Location/Qualifiers
     source          1..6459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    8..24
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13..35
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     32..48
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     32..48
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1281..1297)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1282..1298)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     rep_origin      5517..6105
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6006..6025
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6259..6276
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6393..6414
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6429..6434,6435..6452,6453..6459)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattaccag
       61 atctggctcg agctccaccg cggtggcggc cgctctagag atcgccacta gcgccgcggt
      121 cggaatcagc gcactggccg ccggagcggt cagctacctg ttgctgcgcc gcttcgcttt
      181 ctagcaccga cgtcccggcg ccagcagcta cctctcgagc aaacgttttg cgcgcccgaa
      241 aaataagtcg ttgcagaact ttcatgaatt aggccttgct gcgcccaggc tccagtagta
      301 gaaatggagt cacggcagcc cggtgaagcc aaggtcgaac cggaagagaa ggttcgtcct
      361 cccgacccgg gcacccagca cggcccccgg aacccacgcg gagtcatagc cgcgataatg
      421 gcagaagtgt tgcgggcctg cgtaattgcg aaattcagat ggtgccgacg gccctttggg
      481 tggggctgca gccagaaggg tcgcaaaagc gccgaggcca acccacgcag ccacaaatgc
      541 acgcttggta accgaggatc catggtctcg aagggcgagg aggacaacat ggcgatcatc
      601 aaggagttca tgcgcttcaa ggtccacatg gagggctcgg tcaacggcca cgagttcgag
      661 atcgagggcg agggcgaggg ccgcccgtac gagggcaccc agaccgccaa gctgaaggtc
      721 accaagggcg gcccgctgcc gttcgcctgg gacatcctgt cgccgcagtt catgtacggc
      781 agcaaggcct acgtcaagca cccggccgac atcccggact acctgaagct gtcgttcccg
      841 gagggcttca agtgggagcg cgtcatgaac ttcgaggacg gcggcgtcgt caccgtcacc
      901 caggactcgt cgctgcagga cggcgagttc atctacaagg tcaagctgcg gggcaccaac
      961 ttcccgtcgg acggcccggt catgcagaag aagaccatgg gctgggaggc ctcgtcggag
     1021 cgcatgtacc cggaggacgg cgccctgaag ggcgagatca agcagcggct gaagctgaag
     1081 gacggcggcc actacgacgc cgaggtcaag accacctaca aggccaagaa gccggtccag
     1141 ctgccgggcg cctacaacgt gaacatcaag ctggacatca ccagccacaa cgaggactac
     1201 accatcgtcg agcagtacga gcgcgccgag ggccgccaca gcaccggcgg catggacgag
     1261 ctgtacaagt gaaagcttat cgataccgtc gacctcgagg gggggcccgg tacgtacccg
     1321 gggatcatcg agccgagaac gttatcgaag ttggtcatgt gtaatcccct cgtttgaact
     1381 ttggattaag cgtagataca cccttggaca agccagttgg attcggagac aagcaaattc
     1441 agccttaaaa agggcgaggc ctgcggtggt ggaacaccgc agggcctcta accgctcgac
     1501 gcgctgcacc aaccagcccg cgaacggctg gcagccagcg taaggcgcgg ctcatcgggc
     1561 ggcgttcgcc acgatgtcct gcacttcgag ccaagcctcg aacacctgct ggtgtgcacg
     1621 actcacccgg ttgttgacac cgcgcgcggc cgtgcgggct cggtggggcg gctgtgtcgc
     1681 ccttgccagc gtgagtagcg cgtacctcac ctcgcccaac aggtcgcaca cagccgattc
     1741 gtacgccata aagccaggtg agcccaccag ctccgtaagt tcgggcgctg tgtggctcgt
     1801 acccgcgcat tcaggcggca gggggtctaa cgggtctaag gcggcgtgta cggccgccac
     1861 agcggctctc agcggcccgg aaacgtcctc gaaacgacgc atgtgttcct cctggttggt
     1921 acaggtggtt gggggtgctc ggctgtcgct ggtgttccac caccagggct cgacgggaga
     1981 gcgggggagt gtgcagttgt ggggtggccc ctcagcgaaa tatctgactt ggagctcgtg
     2041 tcggaccata caccggtgat taatcgtggt ctactaccaa gcgtgagcca cgtcgccgac
     2101 gaatttgagc agctctggct gccgtactgg ccgctggcaa gcgacgatct gctcgagggg
     2161 atctaccgcc aaagccgcgc gtcggcccta ggccgccggt acatcgaggc gaacccaaca
     2221 gcgctggcaa acctgctggt cgtggacgta gaccatccag acgcagcgct ccgagcgctc
     2281 agcgcccggg ggtcccatcc gctgcccaac gcgatcgtgg gcaatcgcgc caacggccac
     2341 gcacacgcag tgtgggcact caacgcccct gttccacgca ccgaatacgc gcggcgtaag
     2401 ccgctcgcat acatggcggc gtgcgccgaa ggccttcggc gcgccgtcga cggcgaccgc
     2461 agttactcag gcctcatgac caaaaacccc ggccacatcg cctgggaaac ggaatggctc
     2521 cactcagatc tctacacact cagccacatc gaggccgagc tcggcgcgaa catgccaccg
     2581 ccgcgctggc gtcagcagac cacgtacaaa gcggctccga cgccgctagg gcggaattgc
     2641 gcactgttcg attccgtcag gttgtgggcc tatcgtcccg ccctcatgcg gatctacctg
     2701 ccgacccgga acgtggacgg actcggccgc gcgatctatg ccgagtgcca cgcgcgaaac
     2761 gccgaattcc cgtgcaacga cgtgtgtccc ggaccgctac cggacagcga ggtccgcgcc
     2821 atcgccaaca gcatttggcg ttggatcaca accaagtcgc gcatttgggc ggacgggatc
     2881 gtggtctacg aggccacact cagtgcgcgc cagtcggcca tctcgcggaa gggagcagcg
     2941 cgcacggcgg cgagcacagt tgcgcggcgc gcaaagtccg cgtcagccat ggaggcattg
     3001 ctatgagcga cggctacagc gacggctaca gcgacggcta caaccggcag ccgactgtcc
     3061 gcaaaaagcg gcgcgtgacc gccgccgaag gcgctcgaat caccggacta tccgaacgcc
     3121 acgtcgtccg gctcgtggcg caggaacgca gcgagtggct cgccgagcag gctgcacgcc
     3181 gcgaacgcat ccgcgcctat cacgacgacg agggccactc ttggccgcaa acggccaaac
     3241 atttcgggct gcatctggac accgttaagc gactcggcta tcgggcgagg aaagagcgtg
     3301 cggcagaaca ggaagcggct caaaaggccc acaacgaagc cgacaatcca ccgctgttct
     3361 aacgcaattg gggagcgggt gtcgcggggg ttccgtgggg ggttccgttg caacgggtcg
     3421 gacaggtaaa agtcctggta gacgctagtt ttctggtttg ggccatgcct gtctcgttgc
     3481 gtgtttcgtt gcgcccgttt tgaataccag ccagacgaga cggggttcta cgaatcttgg
     3541 tcgataccaa gccatttccg ctgaatatcg gggagctcac cgccagaatc ggtggttgtg
     3601 gtgatgtacg tggcgaactc cgttgtagtg cctgtggtgg catccgtggc cactctcgtt
     3661 gcacggttcg ttgtgccgtt acaggccccg ttgacagctc accgaacgta gttaaaacat
     3721 gctggtcaaa ctaggtttac caacgatacg agtcagctca tctagggcca gttctaggcg
     3781 ttgttcgttg cgcggttcgt tgcgcatgtt tcgtgtggtt gctagatggc tccgcaacca
     3841 cacgcttcga ggttgagtgc ttccagcacg ggcgcgatcc agaagaactt cgtcgtgcga
     3901 ctgtcctcgt tgatccttgc cgagctggga tggaagctcg gccgaccacc ctggaggaga
     3961 tgatcgagga tgccagggcc tttcacgccc gccgctgctg agcgtccgcc gccgggcccg
     4021 caccgccgtc ggccggcccg ctccgggctc gcagcagcgg gcttcggcgc gggcccgggg
     4081 ctcccgagcg cgggcggggc tccgggcggc cgccgggggc cgggggcggc gccgggcggc
     4141 ccggggcgtc aggcgccggg ggcggtgtcc ggcggccccc agaggaactg cgccagttcc
     4201 tccggatcgg tgaagccgga gagatccagc ggggtctcct cgaacacctc gaagtcgtgc
     4261 aggaaggtga aggcgagcag ttcgcgggcg aagtcctcgg tccgcttcca ctgcgccccg
     4321 tcgagcagcg cggccaggat ctcgcggtcg ccccggaagg cgttgagatg cagttgcacc
     4381 aggctgtagc gggagtctcc cgcatagacg tcggtgaagt cgacgatccc ggtgacctcg
     4441 gtcgcggcca ggtccacgaa gatgttggtc ccgtgcaggt cgccgtggac gaaccggggt
     4501 tcgcggccgg ccagcagcgt gtccacgtcc ggcagccagt cctccaggcg gtccagcagc
     4561 cggggcgaga ggtagcccca cccgcggtgg tcctcgacgg tcgccgcgcg gcgttcccgc
     4621 agcagttccg ggaagacctc ggaatggggg gtgagcacgg tgttcccggt cagcggcacc
     4681 ctgtgcagcc ggccgagcac ccggccgagt tcgcgggcca gggcgagcag cgcgttccgg
     4741 tcggtcgtgc cgtccatcgc ggaccgccag gtggtgccgg tcatccggct catcaccagg
     4801 tagggccacg gccaggctcc ggtgccgggc cgcagctcgc cgcggccgag gaggcggggc
     4861 accggcaccg gggcgtccgc caggaccgcg tacgcctccg actccgacgc gaggctctcc
     4921 ggaccgcacc agtgctcgcc gaacagcttg atcaccgggt cgggctcgcc gaccagtacg
     4981 gggttggtgc tctcgccggg cacccgcagc accggcggca ccggcagccc gagctcctcc
     5041 agggctcggc gggccagcgg ctcccagaat tcctggtcgt tccgcaggct cgcgtaggaa
     5101 tcatccgaat caatacggtc gagaagtaac agggattctt gtgtcacagc ggacctctat
     5161 tcacagggta cgggccggct taattccgca cggccggtcg cgacacggcc tgtccgcacc
     5221 gcggatcagg cgttgacgat gacgggctgg tcggccacgt cggggacgac ggggagtcag
     5281 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5341 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5401 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5461 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5521 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5581 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5641 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     5701 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5761 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5821 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5881 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5941 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6001 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6061 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6121 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6181 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6241 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     6301 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     6361 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     6421 ccccaggctt tacactttat gcttccggct cgtatgttg
//
