LOCUS       sequence-114790-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rab5a with N-terminal FRB and triple myc tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Bentley M, Decker H, Luisi J, Banker G
  TITLE     A novel assay reveals preferential binding between Rabs, kinesins, 
            and specific endosomal subpopulations.
  JOURNAL   J Cell Biol. 2015 Feb 2;208(3):273-281. Epub 2015 Jan 26.
  PUBMED    25624392
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2015-02-2"; volume: "208(3)"; pages: "273-281. Epub 
            2015 Jan 26"
COMMENT     Sequence Label: pBa-FRB-3myc-Rab5a
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..328
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 ctccgcagcc cccaagcttg gcgcgcctag gcctaccatg gcttctagaa tcctctggca
       61 tgagatgtgg catgaaggcc tggaagaggc atctcgtttg tactttgggg aaaggaacgt
      121 gaaaggcatg tttgaggtgc tggagccctt gcatgctatg atggaacggg gcccccagac
      181 tctgaaggaa acatccttta atcaggccta tggtcgagat ttaatggagg cccaagagtg
      241 gtgcaggaag tacatgaaat cagggaatgt caaggacctc ctccaagcct gggacctcta
      301 ttatcatgtg ttccgacgaa tctcaaagac tagttatccg tacggaatgc agaagctgat
      361 ctcagaggag gacctgcaaa aacttatttc tgaagaggat ctgcaaaagc ttatttctga
      421 agaagatctg tacaagcgga ccggaagtgg aatggctaat cgaggagcaa caagacccaa
      481 cgggccaaat actggaaata aaatatgcca gttcaaactg gtccttctag gagagtctgc
      541 tgttggcaaa tcaagcctgg ttcttcgctt tgtgaaaggc caatttcatg aatttcaaga
      601 gagtaccatt ggggctgcct ttctaaccca aactgtgtgt cttgatgaca caacagtaaa
      661 atttgaaata tgggatacag ctggtcaaga acggtatcat agcttagcac caatgtacta
      721 ccgaggagca caagcagcca tagttgtgta tgatatcaca a
//
