LOCUS       sequence-114794-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD Q338R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS501 (TJF040)
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ggccacgaat gcgtccggcg tagaggatcg agatcgatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 actttaagaa ggagatatac catggcattg aagaaggttt ggcttaaccg ttatcccgcg
      181 gacgttccga cggagatcaa ccctgaccgt tatcaatctc tggtagatat gtttgagcag
      241 tcggtcgcgc gctacgccga tcaacctgcg tttgtgaata tgggggaggt aatgaccttc
      301 cgcaagctgg aagaacgcag tcgcgcgttt gccgcttatt tgcaacaagg gttggggctg
      361 aagaaaggcg atcgcgttgc gttgatgatg cctaatttat tgcaatatcc ggtggcgctg
      421 tttggcattt tgcgtgccgg gatgatcgtc gtaaacgtta acccgttgta taccccgcgt
      481 gagcttgagc atcagcttaa cgatagcggc gcatcggcga ttgttatcgt gtctaacttt
      541 gctcacacac tggaaaaagt ggttgataaa accgccgttc agcacgtaat tctgacccgt
      601 atgggcgatc agctatctac ggcaaaaggc acggtagtca atttcgttgt taaatacatc
      661 aagcgtttgg tgccgaaata ccatctgcca gatgccattt catttcgtag cgcactgcat
      721 aacggctacc ggatgcagta cgtcaaaccc gaactggtgc cggaagattt agcttttctg
      781 caatacaccg gcggcaccac tggtgtggcg aaaggcgcga tgctgactca ccgcaatatg
      841 ctggcgaacc tggaacaggt taacgcgacc tatggtccgc tgttgcatcc gggcaaagag
      901 ctggtggtga cggcgctgcc gctgtatcac atttttgccc tgaccattaa ctgcctgctg
      961 tttatcgaac tgggtgggca gaacctgctt atcactaacc cgcgcgatat tccagggttg
     1021 gtaaaagaat tagcgaaata tccgtttacc gctatcacgg gcgttaac
//
