LOCUS       sequence-114797-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD L448S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS509 (TJF032 L448S)
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    75..99
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             114..136
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatcgatc tcgatcccgc gaaattaata
       61 cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt
      121 aactttaaga aggagatata ccatggcatt gaagaaggtt tggcttaacc gttatcccgc
      181 ggacgttccg acggagatca accctgaccg ttatcaatct ctggtagata tgtttgagca
      241 gtcggtcgcg cgctacgccg atcaacctgc gtttgtgaat atgggggagg taatgacctt
      301 ccgcaagctg gaagaacgca gtcgcgcgtt tgccgcttat ttgcaacaag ggttggggct
      361 gaagaaaggc gatcgcgttg cgttgatgat gcctaattta ttgcaatatc cggtggcgct
      421 gtttggcatt ttgcgtgccg ggatgatcgt cgtaaacgtt aacccgttgt ataccccgcg
      481 tgagcttgag catcagctta acgatagcgg cgcatcggcg attgttatcg tgtctaactt
      541 tgctcacaca ctggaaaaag tggttgataa aaccgccgtt cagcacgtaa ttctgacccg
      601 tatgggcgat cagctatcta cggcaaaagg cacggtagtc aatttcgttg ttaaatacat
      661 caagcgtttg gtgccgaaat accatctgcc agatgccatt tcatttcgta gcgcactgca
      721 taacggctac cggatgcagt acgtcaaacc cgaactggtg ccggaagatt tagcttttct
      781 gcaatacacc ggcggcacca ctggtgtggc gaaaggcgcg atgctgactc accgcaatat
      841 gctggcgaac ctggaacagg ttaacgcgac ctatggtccg ctgttgcatc cgggcaaaga
      901 gctggtggtg acggcgctgc cgctgtatca catttttgcc ctgaccatta actgcctgct
      961 gtttatcgaa ctgggtgggc agaacctgct tatcactaac ccgcgcgata ttccagggtt
     1021 ggtaaaagag ttagcgaaa
//
