LOCUS       sequence-114801-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synchronize trafficking of mCherry-GPI from the ER (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: Str-KDEL_SBP-mCherry-GPI
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     152..211
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             212..322
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     primer_bind     complement(474..492)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
       61 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
      121 gataagcttg ccacaacccg ggaggcgcgc catgtacagg atgcaactcc tgtcttgcat
      181 tgcactaagt cttgcacttg tcacgaattc cgacgagaag accactggtt ggcgaggtgg
      241 acacgttgtt gaaggactgg ctggggaact tgaacaactt cgtgcacgac tggagcatca
      301 cccacaaggt caacgtgaac cacctgcagg tgtgagcaag ggcgaggagg ataacatggc
      361 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
      421 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      481 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
      541 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
      601 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
      661 cgtgacccag gactcctccc tacaggacgg cgagttcatc tacaaggtga agctgcgcgg
      721 caccaactt
//
