LOCUS       sequence-114805-         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TNF reporter only (no hook) (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: pCDH_TNF-SBP-EGFP
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..124)
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 tacctgcagg tggttcacgt tgaccttgtg ggtgatgctc cagtcgtgca cgaagttgtt
       61 caagttcccc agccagtcct tcaacaacgt gtccacctcg ccaaccagtg gtcttctcgt
      121 ccatggaatt caaaggccta ggtgcgccat gagctcggcc ctcgaggccc agagcaatga
      181 tgccaaagta gacctggcca gattcggcaa agtcgaggta gtcgggtcga ttgatctcag
      241 cactcagtct atcgcccttc tccagctgga acacgcctcc gaggtagatg ggttcgtacc
      301 aaggtttagc ctcagcgccc tcaggtgttt cccgctgaca tggggatttg atggcagaga
      361 gcagattgac tttagtctgg taagacacgg cgatccgtga aattgtgtgt gtgagcagca
      421 catgtgtaga aggacatccc tggcccttaa acagcacctg ggagtagatg aggtacagtc
      481 cctcactagg cacgacgagc tggttatccc ggagttccac gccattggcg agcagagcgt
      541 ttgccctcct attgagccac tggagctggc cctcggcctg tgggttggcg acgacatggg
      601 cgacaggttt gtcggatgga gtccgagatg aagatcggac agcctgtgcc agtggtgaaa
      661 tgagtgacag at
//
