LOCUS       sequence-114807-        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD H376G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS523 (TJF090)
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    157..181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(181..200)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(182..200)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(289..306)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagacgagtc catgtgctgg cgttcaattt cgcagcagcg gtttctttac cagactcgag
       61 ggtaccgacg tcagcgatcg cgtggccggc cgatatccaa ttgagatctg ccatatgtat
      121 atctccttct tatacttaac taatatacta agatggggaa ttgttatccg ctcacaattc
      181 ccctatagtg agtcgtatta atttcgatta tgcggccgtg tacaatacga ttactttctg
      241 ttcgacttaa gcattatgcg gccgcaagct tggatcctca ttcattcagt gatgatgatg
      301 gtgatgagaa gaacctcagg ctttattgtc cactttgccg cgcgcttcgt cacgtaattc
      361 tcgtcgcaaa atttttccga cgttagattt cggtaactca tcacgaaact ccaccagctt
      421 cggtactttg tagcccgtga gctgacggcg gcaaaaggtc accagtgact cttcggtaag
      481 cgatggatct tttttcacta cgaagatttt caccgcttca ccactggagc cggaaggtac
      541 gccaacagcc gcgacttcct gtacgccagg atgctgcatg acgacatctt caatctcgtt
      601 gggatagacg ttaaaaccgg aaaccagaat catgtctttt ttacgatcga caatgcgcag
      661 gaacccttct tcatccatca ccgcgatgtc gccggtgtgt aaccagccat ttttgatgat
      721 ctcatctgta gcatccggac gctgccagta acccagcatc acctgcggtc ctttgacaca
      781 aagctcaccc ggttgacccg gtggtacttc attatcatca tcatccacca gtttggcttc
      841 cgtcgacggc accggcaaac cgatgcttcc actgccataa tcaatatcat atgggttaac
      901 gctgaccagc ggcgcacact cggtaaggcc atagccttcc agcagatact gtcctgtcag
      961 tttcacccaa cgctctgcca ccacttgctg cactggcatc cctccgcctg cggaaagatg
     1021 cagactggag aaatccagct gctggaactc tttattgttc agcaacgcat tgaacaaggt
     1081 gttaacgcc
//
