LOCUS       sequence-114812-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian retroviral expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Kharas MG, Okabe R, Ganis JJ, Gozo M, Khandan T, Paktinat M, 
            Gilliland DG, Gritsman K
  TITLE     Constitutively active AKT depletes hematopoietic stem cells and 
            induces leukemia in mice.
  JOURNAL   Blood. 2010 Feb 18;115(7):1406-15. doi: 
            10.1182/blood-2009-06-229443. Epub 2009 Dec 14.
  PUBMED    20008787
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2009-06-229443"; journalName: "Blood"; date: 
            "2010-02-18- 18"; volume: "115"; issue: "7"; pages: "1406-15"
COMMENT     Sequence Label: pMSCV-flag-myr-Akt1-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      58..67
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             64..105
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             130..153
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tcagccctca ctccttctct aggcgccgga attagatctc tcgaggttaa cgaattcgcc
       61 accatggggt cttcaaaatc taaaccaaag gaccccagcc agcgccggcg caggatccga
      121 ggttaccttg actacaaaga cgatgacgac aagcaattga gcgacgtggc tattgtgaag
      181 gagggttggc tgcacaaacg aggggagtac atcaagacct ggcggccacg ctacttcctc
      241 ctcaagaatg atggcacctt cattggctac aaggagcggc cgcaggatgt ggaccaacgt
      301 gaggctcccc tcaacaactt ctctgtggcg cagtgccagc tgatgaagac ggagcggccc
      361 cggcccaaca ccttcatcat ccgctgcctg cagtggacca ctgtcatcga acgcaccttc
      421 catgtggaga ctcctgagga gcgggaggag tggacaaccg ccatccagac tgtggctgac
      481 ggcctcaaga agcaggagga ggaggagatg gacttccggt cgggctcacc cagtgacaac
      541 tcaggggctg aagagatgga ggtgtccctg gccaagccca agcaccgcgt gaccatgaac
      601 gagtttgagt acctgaagct gctgggcaag ggcactttcg gcaaggtgat cctggtgaag
      661 gagaaggcca caggccgcta ctacgccatg aagatcctca agaaggaagt catcgtagcc
      721 aaggacgaag tggcccacac actcaccgag aaccgcgtcc tgcagaactc caggcacccc
      781 ttcctcacag ccctgaagta ctctttccag acccacgacc gcctctgctt tgtcatggag
      841 tacgccaacg ggggcgagct gttcttccac ctgtcccggg agcgtgtgtt ctccgaggac
      901 c
//
