LOCUS       sequence-114814-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD T349A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS508 (TJF071)
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    163..187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(187..206)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(188..206)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(295..312)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagtagacga gtccatggtg gctggcgttc aaatttcgca gcagcggttt ctttaccaga
       61 ctcgagggta ccgacgtcag cgatcgcgtg gccggccgat atccaattga gatctgccat
      121 atgtatatct ccttcttata cttaactaat atactaagat ggggaattgt tatccgctca
      181 caattcccct atagtgagtc gtattaattt cgattatgcg gccgtgtaca atacgattac
      241 tttctgttcg acttaagcat tatgcggccg caagcttgga tcctcattca ttcagtgatg
      301 atgatggtga tgagaagaac ctcaggcttt attgtccact ttgccgcgcg cttcgtcacg
      361 taattctcgt cgcaaaattt ttccgacgtt agatttcggt aactcatcac gaaactccac
      421 cagcttcggt actttgtagc ccgtgagctg acggcggcaa aaggtcacca gtgactcttc
      481 ggtaagcgat ggatcttttt tcactacgaa gattttcacc gcttcaccac tggagccgga
      541 aggtacgcca acagccgcga cttcctgtac gccaggatgc tgcatgacga catcttcaat
      601 ctcgttggga tagacgttaa aaccggaaac cagaatcatg tcttttttac gatcgacaat
      661 gcgcaggaac ccttcttcat ccatcaccgc gatgtcgccg gtgtgtaacc agccattttt
      721 gatgatctca tctgtagcat ccggacgctg ccagtaaccc agcatcacct gcggtccttt
      781 gacacaaagc tcacccggtt gacccggtgg tacttcatta tcatcatcat ccaccagttt
      841 ggcttccgtc gacggcaccg gcaaaccgat gctaccacta tgataatcaa tatcatatgg
      901 gttaacgctg accagcggcg cacactcggt aaggccatag ccttccagca gatactgtcc
      961 tgccagtttc acccaacgct ctgccaccac ttgctgcact ggcattcctc cgcctgcgga
     1021 aa
//
